|Positive WB detected in||MCF-7 cells, HEK-293 cells, HeLa cells, mouse heart tissue, mouse brain tissue, rat brain tissue, NIH/3T3 cells, C6 cells|
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
18900-1-AP targets ZO-2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ZO-2 fusion protein Ag13534|
|Full Name||tight junction protein 2 (zona occludens 2)|
|Calculated molecular weight||134 kDa|
|Observed molecular weight||150 kDa|
|GenBank accession number||BC027592|
|Gene ID (NCBI)||9414|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.
What is the molecular weight of ZO-2? Are there any isoforms of ZO-2? Is ZO-2 post-translationally modified?
The molecular weight of ZO-2 is 160 kDa. There are two known isoforms of ZO-2: full-length ZO-2A and ZO-2C lacking the first 23 amino acids (PMID: 10360833). ZO-2 can be phosphorylated and O-glycosylated, which has an impact on its localization (PMID: 23804652).
What is the subcellular localization of ZO-2?
ZO-2 is present at the cytoplasmic face at cell-cell contact sites: tight junctions, adherens junctions, and gap junctions (PMID: 20224657). ZO-2 contains nuclear localization and nuclear export signals and can actively shuttle between the cytoplasm and nucleus (PMID: 11855865). In the nucleus it can affect the activity of various transcription factors. ZO-2 nuclear localization is most prominent in sparse 2D cell cultures and also in cells subjected to mechanical or chemical stress.
What is the tissue expression pattern of ZO-2?
ZO-2 is expressed in various tissues in epithelial and endothelial cells. ZO-2 isoforms have tissue specificity, with ZO-2A being abundant in the heart and brain, while ZO-2C is found in the kidney, pancreas, heart, and placenta (PMID: 11018256).
What proteins can be used as markers of tight junctions?
Zona occludens proteins are often used as tight junction markers. Apart from the antibody against ZO-2, one can use antibodies against ZO-1 (https://www.ptglab.com/products/ZO1-Antibody-21773-1-AP.htm), occludin (https://www.ptglab.com/products/OCLN-Antibody-13409-1-AP.htm), and claudins (e.g., claudin 1: https://www.ptglab.com/products/CLDN1-Antibody-13050-1-AP.htm).
J Therm Biol
Responses of intestinal morphology and function in offspring to heat stress in primiparous sows during late gestation.
Acta Pharmacol Sin
Activation of SIRT1 ameliorates LPS-induced lung injury in mice via decreasing endothelial tight junction permeability.
Biochim Biophys Acta Mol Basis Dis
The zonula occludens protein family regulates the hepatic barrier system in the murine liver.
J Cereb Blood Flow Metab
NLRP3 deficiency ameliorates neurovascular damage in experimental ischemic stroke.
Free Radic Biol Med
Prenylated xanthones from mangosteen (Garcinia mangostana) activate the AhR and Nrf2 pathways and protect intestinal barrier integrity in HT-29 cells.
EMBO Mol Med
Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammation.