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  • KD/KO Validated

Claudin 1 Polyclonal antibody

Claudin 1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13050-1-AP

Synonyms

CLDN1



Tested Applications

Positive WB detected inA431 cells, mouse skin tissue, mouse thymus tissue, mouse liver tissue, A549 cells, rat skin tissue, HUVEC cells
Positive IHC detected inhuman cervical cancer tissue, human malignant melanoma tissue, mouse skin tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells, MDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

13050-1-AP targets Claudin 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse, rat, bovine, canine, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Claudin 1 fusion protein Ag3713
Full Name claudin 1
Calculated molecular weight 211 aa, 23 kDa
Observed molecular weight 20-23 kDa
GenBank accession numberBC012471
Gene symbol CLDN1
Gene ID (NCBI) 9076
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is expressed primarily in keratinocytes and normal mammary epithelial cells.

Protocols

Product Specific Protocols
WB protocol for Claudin 1 antibody 13050-1-APDownload protocol
IHC protocol for Claudin 1 antibody 13050-1-APDownload protocol
IF protocol for Claudin 1 antibody 13050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC

Clin Cancer Res

ZIP4 Promotes Pancreatic Cancer Progression by Repressing ZO-1 and claudin-1 through a ZEB1-Dependent Transcriptional Mechanism.

Authors - Mingyang Liu
humanIF

EMBO Rep

O-glycan initiation directs distinct biological pathways and controls epithelial differentiation.

Authors - Ieva Bagdonaite
humanWB

PLoS Pathog

Signal Peptidase Complex Subunit 1 Participates in the Assembly of Hepatitis C Virus through an Interaction with E2 and NS2.

Authors - Suzuki Ryosuke R
mouseWB,IHC

Gut Microbes

Host Gasdermin D restrains systemic endotoxemia by capturing Proteobacteria in the colon of high-fat diet-feeding mice.

Authors - Yujie Shi
mouseWB

Gut Microbes

Microbial hydrogen economy alleviates colitis by reprogramming colonocyte metabolism and reinforcing intestinal barrier.

Authors - Li Ge
mouseWB

Elife

Post-transcriptional repression of circadian component CLOCK regulates cancer-stemness in murine breast cancer cells.

Authors - Takashi Ogino

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

James (Verified Customer) (01-06-2022)

Works good. Nice image

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Gut
Claudin 1 Antibody Immunohistochemistry validation (1:100 dilution) in Gut (Cat no:13050-1-AP)
FH

Joshua (Verified Customer) (07-27-2019)

Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Moderate staining with background. Staining is a mix of junctional (bright) and cytosolic (dim).

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human Keratinocytes
Claudin 1 Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:13050-1-AP)