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Claudin 1 Polyclonal antibody
Claudin 1 Polyclonal Antibody for IF, IHC, WB, ELISA
Cat no : 13050-1-AP
Validation Data Gallery
|Positive WB detected in||A431 cells, mouse skin tissue, mouse thymus tissue, mouse liver tissue, rat skin tissue, A549 cells, HUVEC cells|
|Positive IHC detected in||human cervical cancer tissue, human malignant melanoma tissue, mouse skin tissue, human oesophagus cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HUVEC cells, MDCK cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:1000-1:4000|
|Sample-dependent, check data in validation data gallery|
13050-1-AP targets Claudin 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
|Tested Reactivity||human, mouse, rat, canine|
|Cited Reactivity||human, mouse, rat, chicken, sheep, pig, canine, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Claudin 1 fusion protein Ag3713|
|Full Name||claudin 1|
|Calculated molecular weight||211 aa, 23 kDa|
|Observed molecular weight||20-23 kDa|
|GenBank accession number||BC012471|
|Gene ID (NCBI)||9076|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is expressed primarily in keratinocytes and normal mammary epithelial cells.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
James (Verified Customer) (01-06-2022)
Works good. Nice image
Joshua (Verified Customer) (07-27-2019)
Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Moderate staining with background. Staining is a mix of junctional (bright) and cytosolic (dim).