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- KD/KO Validated
Claudin 1 Polyclonal antibody
Claudin 1 Polyclonal Antibody for IF, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, canine and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13050-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, A549 cells, mouse liver tissue, mouse skin tissue, mouse thymus tissue, HUVEC cells, rat skin tissue |
| Positive IF detected in | HUVEC cells, MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF) | IF : 1:1000-1:4000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 71 publications below |
| IHC | See 13 publications below |
| IF | See 12 publications below |
Product Information
13050-1-AP targets Claudin 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| Tested Reactivity | human, mouse, rat, canine |
| Cited Reactivity | bovine, canine, human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Claudin 1 fusion protein Ag3713 |
| Full Name | claudin 1 |
| Calculated molecular weight | 211 aa, 23 kDa |
| Observed molecular weight | 20-23 kDa |
| GenBank accession number | BC012471 |
| Gene symbol | CLDN1 |
| Gene ID (NCBI) | 9076 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is expressed primarily in keratinocytes and normal mammary epithelial cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Claudin 1 antibody 13050-1-AP | Download protocol |
| IF protocol for Claudin 1 antibody 13050-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Modulating the gut microbiota and inflammation is involved in the effect of Bupleurum polysaccharides against diabetic nephropathy in mice. | ||
Biochim Biophys Acta Gene Regul Mech The chromatin remodeling protein BRM regulates the transcription of tight junction proteins: Implication in breast cancer metastasis. | ||
Biochem Biophys Res Commun Bacterial β-glucuronidase alleviates dextran sulfate sodium-induced colitis in mice: A possible crucial new diagnostic and therapeutic target for inflammatory bowel disease. | ||
Microorganisms Oral Administration of a Select Mixture of Lactobacillus and Bacillus Alleviates Inflammation and Maintains Mucosal Barrier Integrity in the Ileum of Pigs Challenged with Salmonella Infantis. | ||
Int Immunopharmacol (+)-Borneol improves the efficacy of edaravone against DSS-induced colitis by promoting M2 macrophages polarization via JAK2-STAT3 signaling pathway. | ||
J Agric Food Chem The response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early-life galactooligosaccharides intervention in suckling piglets. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Joshua (Verified Customer) (07-27-2019) | Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Moderate staining with background. Staining is a mix of junctional (bright) and cytosolic (dim).
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