Claudin 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Claudin 1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

121

Reviews (2)

Cat no : 13050-1-AP

Synonyms

CLDN1

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Validation Data Gallery

• 

  WB analysis using 13050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 13050-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13050-1-AP (CLDN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 13050-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13050-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13050-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 13050-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 13050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using Claudin 1 antibody (13050-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  IF Staining of MDCK using 13050-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 13050-1-AP (Claudin 1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, mouse skin tissue, mouse thymus tissue, mouse liver tissue, A549 cells, rat skin tissue, HUVEC cells
• Positive IHC detected in: human cervical cancer tissue, human malignant melanoma tissue, human oesophagus cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HUVEC cells, MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:1000-1:4000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 100 publications below
• IHC: See 15 publications below
• IF: See 15 publications below

Product Information

13050-1-AP targets Claudin 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse, rat, bovine, canine, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Claudin 1 fusion protein Ag3713
• Full Name: claudin 1
• Calculated molecular weight: 211 aa, 23 kDa
• Observed molecular weight: 20-23 kDa
• GenBank accession number: BC012471
• Gene symbol: CLDN1
• Gene ID (NCBI): 9076
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is expressed primarily in keratinocytes and normal mammary epithelial cells.

Publications

Species	Application	Title
mouse	WB	Int J Biol Macromol; Modulating the gut microbiota and inflammation is involved in the effect of Bupleurum polysaccharides against diabetic nephropathy in mice.; Authors - Yuchen Feng
human	WB	Biochim Biophys Acta Gene Regul Mech; The chromatin remodeling protein BRM regulates the transcription of tight junction proteins: Implication in breast cancer metastasis.; Authors - Yuyu Yang
mouse	WB	Biochem Biophys Res Commun; Bacterial β-glucuronidase alleviates dextran sulfate sodium-induced colitis in mice: A possible crucial new diagnostic and therapeutic target for inflammatory bowel disease.; Authors - Yan He
pig	WB	Microorganisms; Oral Administration of a Select Mixture of Lactobacillus and Bacillus Alleviates Inflammation and Maintains Mucosal Barrier Integrity in the Ileum of Pigs Challenged with Salmonella Infantis.; Authors - Xiao Liu
human	IHC	Int Immunopharmacol; (+)-Borneol improves the efficacy of edaravone against DSS-induced colitis by promoting M2 macrophages polarization via JAK2-STAT3 signaling pathway.; Authors - Xiong Zhang
pig	WB	J Agric Food Chem; The response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early-life galactooligosaccharides intervention in suckling piglets.; Authors - Jue Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

James (Verified Customer) (01-06-2022)

Works good. Nice image

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Gut

Claudin 1 Antibody Immunohistochemistry validation (1:100 dilution) in Gut (Cat no:13050-1-AP)

FH

Joshua (Verified Customer) (07-27-2019)

Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Moderate staining with background. Staining is a mix of junctional (bright) and cytosolic (dim).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human Keratinocytes

Claudin 1 Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:13050-1-AP)

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