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Claudin 1 Antibody 13 Publications

Rabbit Polyclonal| Catalog number: 13050-1-AP

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

Claudin 1 Antibody WB mouse skin tissue 13050-1-AP

mouse skin tissue were subjected to SDS PAGE followed by western blot with 13050-1-AP( Claudin 1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Claudin 1 Antibody IF MDCK cells 13050-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed MDCK cells using 13050-1-AP (Claudin 1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Claudin 1 Antibody IHC human skin tissue 13050-1-AP

Immunohistochemical of paraffin-embedded human skin using 13050-1-AP(CLDN1 antibody) at dilution of 1:50 (under 40x lens)

Species specificity:
human, mouse, dog, swine
Positive WB detected in:
mouse skin tissue, mouse liver tissue, mouse thymus tissue
Positive IHC detected in:
human skin tissue, human cervical cancer tissue, human liver tissue, human oesophagus cancer tissue, human skin cancer tissue, mouse colon tissue
Positive IF detected in:
MDCK cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Claudin 1 fusion protein Ag3713
Full name:
claudin 1
Calculated molecular weight:
211aa,23 kDa
Observed molecular weight:
23 kDa
GenBank accession number:
BC012471
Gene ID (NCBI):
9076
Gene symbol
CLDN1
Synonyms
claudin 1, CLD1, CLDN1, ILVASC, SEMP1

Background

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is expressed primarily in keratinocytes and normal mammary epithelial cells.


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