|Positive WB detected in||A431 cells, mouse skin tissue, mouse thymus tissue, A549 cells, mouse liver tissue, HUVEC cells|
|Positive IF detected in||HUVEC cells, MDCK cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13050-1-AP targets Claudin 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, dog samples.
|Tested Reactivity||human, mouse, dog|
|Cited Reactivity||canine, dog, human, mouse, swine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Claudin 1 fusion protein Ag3713|
|Full Name||claudin 1|
|Calculated molecular weight||211 aa, 23 kDa|
|Observed molecular weight||20-23 kDa|
|GenBank accession number||BC012471|
|Gene ID (NCBI)||9076|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is expressed primarily in keratinocytes and normal mammary epithelial cells.
Int J Biol Macromol
Modulating the gut microbiota and inflammation is involved in the effect of Bupleurum polysaccharides against diabetic nephropathy in mice.
Biochim Biophys Acta Gene Regul Mech
The chromatin remodeling protein BRM regulates the transcription of tight junction proteins: Implication in breast cancer metastasis.
Biochem Biophys Res Commun
Bacterial β-glucuronidase alleviates dextran sulfate sodium-induced colitis in mice: A possible crucial new diagnostic and therapeutic target for inflammatory bowel disease.
Oral Administration of a Select Mixture of Lactobacillus and Bacillus Alleviates Inflammation and Maintains Mucosal Barrier Integrity in the Ileum of Pigs Challenged with Salmonella Infantis.
(+)-Borneol improves the efficacy of edaravone against DSS-induced colitis by promoting M2 macrophages polarization via JAK2-STAT3 signaling pathway.
J Agric Food Chem
The response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to early-life galactooligosaccharides intervention in suckling piglets.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joshua (Verified Customer) (07-27-2019)
Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Moderate staining with background. Staining is a mix of junctional (bright) and cytosolic (dim).