Claudin 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Claudin 1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (5)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

271

Reviews (2)

Cat no : 13050-1-AP

Synonyms

Caudin 1, CLDN1, SEMP1, ILVASC, CLD1

---

Validation Data Gallery

• 

  WB analysis of A431 using 13050-1-AP

  WB result of Claudin 1 antibody (13050-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-Claudin 1 transfected A431 cells.

• 

  WB analysis using 13050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13050-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 13050-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13050-1-AP (CLDN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 13050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 13050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using Claudin 1 antibody (13050-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  IF Staining of MDCK using 13050-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 13050-1-AP (Claudin 1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: A431 cells, mouse liver tissue, mouse skin tissue, mouse thymus tissue, A549 cells, HUVEC cells, rat skin tissue
• Positive IHC detected in: human cervical cancer tissue, human skin cancer tissue, mouse skin tissue, human oesophagus cancer tissue, human malignant melanoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HUVEC cells, MDCK cells

Freshly prepared samples are recommended.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 213 publications below
• IHC: See 47 publications below
• IF: See 48 publications below

Product Information

13050-1-AP targets Claudin 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse, rat, pig, canine, chicken, bovine, sheep, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Claudin 1 fusion protein Ag3713
• Full Name: claudin 1
• Calculated Molecular Weight: 211 aa, 23 kDa
• Observed Molecular Weight: 20-23 kDa
• GenBank Accession Number: BC012471
• Gene Symbol: Claudin 1
• Gene ID (NCBI): 9076
• RRID: AB_2079881
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with similar structures. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is an integral membrane protein expressed primarily in keratinocytes and normal mammary epithelial cells. Claudin 1 forms tight junctions with other claudin proteins and plays an important role in the intestinal epithelial barrier.

Publications

Species	Application	Title
mouse	IHC	Cell Host Microbe; Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation; Authors - Zhenyu Tian
mouse	IF	J Nanobiotechnology; Orally biomimetic metal-phenolic nanozyme with quadruple safeguards for intestinal homeostasis to ameliorate ulcerative colitis; Authors - Yuanyuan Zhu
human,mouse	IHC	Clin Cancer Res; ZIP4 Promotes Pancreatic Cancer Progression by Repressing ZO-1 and claudin-1 through a ZEB1-Dependent Transcriptional Mechanism.; Authors - Mingyang Liu
human	IF	EMBO Rep; O-glycan initiation directs distinct biological pathways and controls epithelial differentiation.; Authors - Ieva Bagdonaite
mouse	IF	Int J Biol Macromol; Glucose-modified BSA/procyanidin C1 NPs penetrate the blood-brain barrier and alleviate neuroinflammation in Alzheimer's disease models; Authors - Linyan Duan
rat	IHC	Mater Today Bio; Intracellular hydrogelation of macrophage conjugated probiotics for hitchhiking delivery and combined treatment of colitis; Authors - Jingzhe Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

James (Verified Customer) (01-06-2022)

Works good. Nice image

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Gut

Claudin 1 Antibody Immunohistochemistry validation (1:100 dilution) in Gut (Cat no:13050-1-AP)

FH

Joshua (Verified Customer) (07-27-2019)

Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Moderate staining with background. Staining is a mix of junctional (bright) and cytosolic (dim).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human Keratinocytes

Claudin 1 Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:13050-1-AP)

Related Products

Claudin 3 Polyclonal antibody

16456-1-AP

Claudin 4-specific Polyclonal antibody

16195-1-AP

ZO-1 Polyclonal antibody

21773-1-AP

ZO-2 Polyclonal antibody

18900-1-AP

Claudin 11 Polyclonal antibody

12152-1-AP