Various lysates were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours

A431 cells were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

A549 cells were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

mouse liver tissue were subjected to SDS PAGE followed by western blot with 13050-1-AP( Claudin 1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

mouse skin tissue were subjected to SDS PAGE followed by western blot with 13050-1-AP( Claudin 1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13050-1-AP(CLDN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Immunofluorescent analysis of ( 4% PFA) fixed HUVEC cells using 13050-1-AP (Claudin 1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent analysis of ( -20°C Ethanol) fixed MDCK cells using 13050-1-AP (Claudin 1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)