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Claudin 1 Antibody 21 Publications

Rabbit Polyclonal| Catalog number: 13050-1-AP

1 customer review

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

Claudin 1 Antibody WB mouse skin tissue 13050-1-AP

mouse skin tissue were subjected to SDS PAGE followed by western blot with 13050-1-AP( Claudin 1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Claudin 1 Antibody IF MDCK cells 13050-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed MDCK cells using 13050-1-AP (Claudin 1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, dog, canine, swine
Positive WB detected in:
mouse skin tissue, mouse thymus tissue, A431 cells, A549 cells, mouse liver tissue
Positive IF detected in:
MDCK cells
Recommended dilution:
WB : 1:500-1:1000
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Claudin 1 fusion protein Ag3713
Full name:
claudin 1
Calculated molecular weight:
211aa,23 kDa
Observed molecular weight:
23 kDa
GenBank accession number:
BC012471
Gene ID (NCBI):
9076
Gene symbol
CLDN1
Synonyms
claudin 1, CLD1, CLDN1, ILVASC, SEMP1

Background

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is expressed primarily in keratinocytes and normal mammary epithelial cells.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

Claudin 1 Rabbit Polyclonal antibody

Joshua (Verified Customer), 07-27-2019
Applications
Immunofluorescence,
Primary Antibody Dilution
1:500
Claudin 1 Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:13050-1-AP)

Claudin 1 Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:13050-1-AP)

Cell/Tissue Type
Human Keratinocytes
Lot Number
Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Moderate staining with background. Staining is a mix of junctional (bright) and cytosolic (dim).

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