• Featured Product
  • KD/KO Validated

TLR4 Monoclonal antibody

TLR4 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

3G9A4

Cat no : 66350-1-Ig

Synonyms

ARMD10, CD284, hToll, TLR4, TOLL, toll like receptor 4



Tested Applications

Positive WB detected inRAW 264.7 cells, pig liver tissue, NIH/3T3 cells, rat liver tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66350-1-Ig targets TLR4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TLR4 fusion protein Ag13857
Full Name toll-like receptor 4
Calculated molecular weight 839 aa, 96 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC117422
Gene symbol TLR4
Gene ID (NCBI) 7099
RRIDAB_2881730
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.

Protocols

Product Specific Protocols
WB protocol for TLR4 antibody 66350-1-IgDownload protocol
IHC protocol for TLR4 antibody 66350-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation

Authors - Chuanlong Wang
ratWB

Acta Biomater

An injectable co-assembled hydrogel blocks reactive oxygen species and inflammation cycle resisting myocardial ischemia-reperfusion injury.

Authors - Xu Liao
mouseWB

Cell Rep

METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling

Authors - Shuhua Luo
pigWB

Int J Biol Macromol

Angelica sinensis aboveground part polysaccharide and its metabolite 5-MT ameliorate colitis via modulating gut microbiota and TLR4/MyD88/NF-κB pathway

Authors - Yuan-Feng Zou
mouseWB

Gut Microbes

Microbial hydrogen economy alleviates colitis by reprogramming colonocyte metabolism and reinforcing intestinal barrier.

Authors - Li Ge
human,mouseWB

J Control Release

Inhalation of MSC-EVs is a noninvasive strategy for ameliorating acute lung injury.

Authors - Ruijing Zhao