TLR4 Monoclonal antibody

TLR4 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b


human, mouse, rat, pig







Cat no : 66350-1-Ig


ARMD10, CD284, hToll, TLR4, TOLL, toll like receptor 4

Tested Applications

Positive WB detected inRAW 264.7 cells, pig liver tissue, NIH/3T3 cells, rat liver tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66350-1-Ig targets TLR4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TLR4 fusion protein Ag13857
Full Name toll-like receptor 4
Calculated molecular weight 839 aa, 96 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC117422
Gene symbol TLR4
Gene ID (NCBI) 7099
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.


Product Specific Protocols
WB protocol for TLR4 antibody 66350-1-IgDownload protocol
IHC protocol for TLR4 antibody 66350-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Bioeng Biotechnol

Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.

Authors - Kaile Zhang

Exp Biol Med (Maywood)

Oxymatrine suppresses IL-1β-induced degradation of the nucleus pulposus cell and extracellular matrix through the TLR4/NF-κB signaling pathway.

Authors - Kang Wei

Stem Cells Int

EZH2 Regulates Lipopolysaccharide-Induced Periodontal Ligament Stem Cell Proliferation and Osteogenesis through TLR4/MyD88/NF-κB Pathway.

Authors - Pengcheng Wang

Pak J Pharm Sci

Platycodon grandiflorum extract attenuates lipopolysaccharide-induced acute lung injury via TLR4/NF-κBp65 pathway in rats.

Authors - Li Si-Cong

Exp Ther Med

Effect of HMGB1 on monocyte immune function in respiratory syncytial virus bronchiolitis.

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Evid Based Complement Alternat Med

Electroacupuncture Alleviates Postoperative Cognitive Dysfunction in Aged Rats by Inhibiting Hippocampal Neuroinflammation Activated via Microglia/TLRs Pathway.

Authors - Pei-Pei Feng