- Featured Product
- KD/KO Validated
TLR4 Monoclonal antibody
TLR4 Monoclonal Antibody for IHC, WB, ELISA
Cat no : 66350-1-Ig
|Positive WB detected in
|RAW 264.7 cells, pig liver tissue, NIH/3T3 cells, rat liver tissue
|Positive IHC detected in
|human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:1000-1:8000
|IHC : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66350-1-Ig targets TLR4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|human, mouse, rat, pig
|human, mouse, rat, pig
|Host / Isotype
|Mouse / IgG2b
|TLR4 fusion protein Ag13857
|toll-like receptor 4
|Calculated molecular weight
|839 aa, 96 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.
Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation
An injectable co-assembled hydrogel blocks reactive oxygen species and inflammation cycle resisting myocardial ischemia-reperfusion injury.
METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling
Int J Biol Macromol
Angelica sinensis aboveground part polysaccharide and its metabolite 5-MT ameliorate colitis via modulating gut microbiota and TLR4/MyD88/NF-κB pathway
Microbial hydrogen economy alleviates colitis by reprogramming colonocyte metabolism and reinforcing intestinal barrier.
J Control Release
Inhalation of MSC-EVs is a noninvasive strategy for ameliorating acute lung injury.