TMED5 Polyclonal antibody

TMED5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30393-1-AP

Synonyms

CGI 100, DKFZp781B2248, TMED5, UNQ397/PRO733

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Validation Data Gallery

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  WB analysis using 30393-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30393-1-AP (TMED5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, MKN-45 cells, NCI-H1299 cells, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30393-1-AP targets TMED5 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMED5 fusion protein Ag33253
• Full Name: transmembrane emp24 protein transport domain containing 5
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC070051
• Gene Symbol: TMED5
• Gene ID (NCBI): 50999
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMED5 ( transmembrane p24 trafficking protein 5) is highly expressed incervical and bladder cancer cell lines. TMED5 promotes nuclear autophagy and the malignant behavior of cervical cancer cells (PMID:36530030). Moreover, TMED5 could interact with WNT7B and thus activated the canonical WNT-CTNNB1/β-catenin signaling pathway (PMID:30394198).