Tested Applications
| Positive WB detected in | A549 cells, pig liver tissue, HeLa cells, HepG2 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21620-1-AP targets TMED9 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13813 Product name: Recombinant human TMED9 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-224 aa of BC001123 Sequence: MAVELGVLLVRPRPGTGLGRVMRTLLLVLWLATRGSALYFHIGETEKKCFIEEIPDETMVIGNYRTQLYDKQREEYQPATPGLGMFVEVKDPEDKVILARQYGSEGRFTFTSHTPGEHQICLHSNSTKFSLFAGGMLRVHLDIQVGEHANDYAEIAAKDKLSELQLRVRQLVEQVEQIQKEQNYQRWREERFRQTSESTNQRVLWWSILQTLILVAIGVWQMRH Predict reactive species |
| Full Name | transmembrane emp24 protein transport domain containing 9 |
| Calculated Molecular Weight | 235 aa, 27 kDa |
| Observed Molecular Weight | 25-27 kDa |
| GenBank Accession Number | BC001123 |
| Gene Symbol | TMED9 |
| Gene ID (NCBI) | 54732 |
| RRID | AB_10858623 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BVK6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TMED9 (also named as GMP25, GP25L2 or p25) is a member of the EMP24/GP25L family. TMED9 probably forms hetero-oligomeric complexes with TMED7 (p27), TMED2 (p24), and TMED10 (p23) (PMID: 10359607). Located in the endoplasmic reticulum and the Golgi, TMED9 appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway (PMID: 9472029; 10359607).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TMED9 antibody 21620-1-AP | Download protocol |
| IHC protocol for TMED9 antibody 21620-1-AP | Download protocol |
| IP protocol for TMED9 antibody 21620-1-AP | Download protocol |
| WB protocol for TMED9 antibody 21620-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody is anti-TMED9 (Cat# 21620-1-AP), with a total of 11 citations. Citations appear in journals including Cell, Cell Research, Advanced Science, Frontiers in Immunology, PLoS Biology, Journal of Cell Biology, BMC Biology, Annals of Translational Medicine, Molecular Biology of the Cell, and Bioengineered. Research fields span unconventional protein secretion, ER-to-Golgi trafficking, autophagosome biogenesis, lysosomal degradation, ER stress, and cancer biology (glioma, breast carcinoma).
| Species | Application | Title |
|---|---|---|
Cell A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.
| ||
Cell Small Molecule Targets TMED9 and Promotes Lysosomal Degradation to Reverse Proteinopathy. | ||
Cell Res A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.
| ||
Adv Sci (Weinh) TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike. | ||
Front Immunol TMED9: a potential therapeutic target and prognostic marker in glioma and its implications across pan-cancer contexts | ||
PLoS Biol TMED9 coordinates the clearance of misfolded GPI-anchored proteins out of the ER and into the Golgi |
Reviews
What customers say
Based on one review, this antibody is rated 4/5 and described as specific, performing well in Western blot for both cell and tissue lysates. The reviewer also used it for immunofluorescence. Overall, users report reliable specificity and good performance across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Giulia (Verified Customer) (02-29-2024) | Specific antibody that works well in Western blot, both for cells and tissue lysates.
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