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TMED9 Polyclonal antibody
TMED9 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 21620-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, L02 cells, pig liver tissue, mouse lung tissue, mouse pancreas tissue, mouse brain tissue, mouse liver tissue, rat liver tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells, HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 4 publications below |
WB | See 6 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
CoIP | See 1 publications below |
Product Information
21620-1-AP targets TMED9 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TMED9 fusion protein Ag13813 |
Full Name | transmembrane emp24 protein transport domain containing 9 |
Calculated molecular weight | 235 aa, 27 kDa |
Observed molecular weight | 24-27 kDa |
GenBank accession number | BC001123 |
Gene symbol | TMED9 |
Gene ID (NCBI) | 54732 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TMED9 (also named as GMP25, GP25L2 or p25) is a member of the EMP24/GP25L family. TMED9 probably forms hetero-oligomeric complexes with TMED7 (p27), TMED2 (p24), and TMED10 (p23) (PMID: 10359607). Located in the endoplasmic reticulum and the Golgi, TMED9 appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway (PMID: 9472029; 10359607).
Protocols
Product Specific Protocols | |
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WB protocol for TMED9 antibody 21620-1-AP | Download protocol |
IHC protocol for TMED9 antibody 21620-1-AP | Download protocol |
IF protocol for TMED9 antibody 21620-1-AP | Download protocol |
IP protocol for TMED9 antibody 21620-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Small Molecule Targets TMED9 and Promotes Lysosomal Degradation to Reverse Proteinopathy. | ||
Cell A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.
| ||
Cell Res A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.
| ||
BMC Biol ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier. | ||
Bioengineered High expression of transmembrane P24 trafficking protein 9 predicts poor prognosis in breast carcinoma.
| ||
Adv Sci (Weinh) TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike. |