TMEM106B Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TMEM106B Monoclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5D1F8

Cat no : 60333-1-PBS

Synonyms

TMEM106B, transmembrane protein 106B

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Validation Data Gallery

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  WB analysis using 60333-1-Ig (same clone as 60333-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60333-1-Ig (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60333-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60333-1-Ig (same clone as 60333-1-PBS)

  WB result of TMEM106B antibody (60333-1-Ig; 1:2000; room temperature for 1.5 hours) with wild-type and TMEM106B knockout HeLa cells. This data was developed using the same antibody clone with 60333-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60333-1-Ig (same clone as 60333-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60333-1-Ig (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60333-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60333-1-Ig (same clone as 60333-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60333-1-Ig (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60333-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 60333-1-Ig (same clone as 60333-1-PBS)

  IP result of anti-TMEM106B (IP:60333-1-Ig, 5ug; Detection:60333-1-Ig 1:500) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 60333-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60333-1-PBS targets TMEM106B in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TMEM106B fusion protein Ag21448
• Full Name: transmembrane protein 106B
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: ~40 kDa
• GenBank Accession Number: BC033901
• Gene Symbol: TMEM106B
• Gene ID (NCBI): 54664
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TMEM106B is a genetic risk factor for frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP). Amyotrophic lateral sclerosis (ALS), like FTLD-TDP, is characterized by pathological TDP-43 inclusions. TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression.