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TMEM106B Monoclonal antibody

TMEM106B Monoclonal Antibody for IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5D1F8

Cat no : 60333-1-Ig

Synonyms

TMEM106B, transmembrane protein 106B



Tested Applications

Positive WB detected inU2OS cells, HeLa cells, K-562 cells, LNCaP cells, A549 cells, Jurkat cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 2 publications below

Product Information

60333-1-Ig targets TMEM106B in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TMEM106B fusion protein Ag21448
Full Name transmembrane protein 106B
Calculated molecular weight 31 kDa
Observed molecular weight ~40 kDa
GenBank accession numberBC033901
Gene symbol TMEM106B
Gene ID (NCBI) 54664
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM106B is a genetic risk factor for frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP). Amyotrophic lateral sclerosis (ALS), like FTLD-TDP, is characterized by pathological TDP-43 inclusions. TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression.

Protocols

Product Specific Protocols
WB protocol for TMEM106B antibody 60333-1-IgDownload protocol
IP protocol for TMEM106B antibody 60333-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell

Homotypic fibrillization of TMEM106B across diverse neurodegenerative diseases.

Authors - Andrew Chang
humanIHC

Acta Neuropathol

Accumulation of TMEM106B C-terminal fragments in neurodegenerative disease and aging

Authors - Jolien Perneel