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TMEM106B Monoclonal antibody
TMEM106B Monoclonal Antibody for IP, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
5D1F8
Cat no : 60333-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | U2OS cells, HeLa cells, K-562 cells, LNCaP cells, A549 cells, Jurkat cells |
Positive IP detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
IHC | See 2 publications below |
Product Information
60333-1-Ig targets TMEM106B in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | TMEM106B fusion protein Ag21448 |
Full Name | transmembrane protein 106B |
Calculated molecular weight | 31 kDa |
Observed molecular weight | ~40 kDa |
GenBank accession number | BC033901 |
Gene symbol | TMEM106B |
Gene ID (NCBI) | 54664 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TMEM106B is a genetic risk factor for frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP). Amyotrophic lateral sclerosis (ALS), like FTLD-TDP, is characterized by pathological TDP-43 inclusions. TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for TMEM106B antibody 60333-1-Ig | Download protocol |
IP protocol for TMEM106B antibody 60333-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Brain C-terminal TMEM106B fragments in human brain correlate with disease-associated TMEM106B haplotypes | ||
Acta Neuropathol Accumulation of TMEM106B C-terminal fragments in neurodegenerative disease and aging | ||
Front Cell Neurosci Loss of TMEM106B exacerbates C9ALS/FTD DPR pathology by disrupting autophagosome maturation
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