TMEM106B (1-46aa) Monoclonal antibody

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  WB analysis using 60333-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60333-1-Ig (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60333-1-Ig

  WB result of TMEM106B antibody (60333-1-Ig; 1:2000; room temperature for 1.5 hours) with wild-type and TMEM106B knockout HeLa cells.

• 

  WB analysis of HeLa using 60333-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60333-1-Ig (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60333-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60333-1-Ig (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 60333-1-Ig

  IP result of anti-TMEM106B (IP:60333-1-Ig, 5ug; Detection:60333-1-Ig 1:500) with HeLa cells lysate 1720 ug.

• Featured Product
• KD/KO Validated

TMEM106B (1-46aa) Monoclonal Antibody for WB, IP, ELISA

Cat No. 60333-1-Ig

Clone No.5D1F8

Publications(7)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IP, ELISA

Synonyms

TMEM106B, 5D1F8, Transmembrane protein 106B

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Tested Applications

• Positive WB detected in: U2OS cells, HeLa cells, K-562 cells, LNCaP cells, A549 cells, Jurkat cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

60333-1-Ig targets TMEM106B (1-46aa) in WB, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TMEM106B (1-46aa) fusion protein Ag21448
• Full Name: transmembrane protein 106B
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: ~40 kDa
• GenBank Accession Number: BC033901
• Gene Symbol: TMEM106B
• Gene ID (NCBI): 54664
• RRID: AB_2881442
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9NUM4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM106B is a genetic risk factor for frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP). Amyotrophic lateral sclerosis (ALS), like FTLD-TDP, is characterized by pathological TDP-43 inclusions. TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression.

Publications

Species	Application	Title
human		Cell; Homotypic fibrillization of TMEM106B across diverse neurodegenerative diseases.; Authors - Andrew Chang
human	WB	Brain; C-terminal TMEM106B fragments in human brain correlate with disease-associated TMEM106B haplotypes; Authors - Cristina T Vicente
human	WB	Pathol Res Pract; TESC acts as a prognostic factor and promotes epithelial-mesenchymal transition progression in esophageal squamous carcinoma; Authors - Yanxin Dong
human	WB,IP,IF	F1000Res; The identification of high-performing antibodies for transmembrane protein 106B (TMEM106B) for use in Western blot, immunoprecipitation, and immunofluorescence; Authors - Riham Ayoubi ; KO Validated
human	WB	FASEB J; Lysosomal membrane protein TMEM106B modulates hematopoietic stem and progenitor cell proliferation and differentiation by regulating LAMP2A stability; Authors - Di Guo
human		Acta Neuropathol; Accumulation of TMEM106B C-terminal fragments in neurodegenerative disease and aging; Authors - Jolien Perneel