TMEM106B Monoclonal antibody

• Featured Product
• KD/KO Validated

TMEM106B Monoclonal Antibody for IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

5D1F8

Cat no : 60333-1-Ig

Synonyms

TMEM106B, transmembrane protein 106B

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Validation Data Gallery

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  WB analysis using 60333-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60333-1-Ig (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60333-1-Ig

  WB result of TMEM106B antibody (60333-1-Ig; 1:2000; room temperature for 1.5 hours) with wild-type and TMEM106B knockout HeLa cells.

• 

  WB analysis of HeLa using 60333-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60333-1-Ig (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60333-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60333-1-Ig (TMEM106B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 60333-1-Ig

  IP result of anti-TMEM106B(IP:60333-1-Ig, 5ug; Detection:60333-1-Ig 1:500) with HeLa cells lysate 1720 ug.

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Tested Applications

• Positive WB detected in: U2OS cells, HeLa cells, K-562 cells, LNCaP cells, A549 cells, Jurkat cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

60333-1-Ig targets TMEM106B in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TMEM106B fusion protein Ag21448
• Full Name: transmembrane protein 106B
• Calculated molecular weight: 31 kDa
• Observed molecular weight: ~40 kDa
• GenBank accession number: BC033901
• Gene symbol: TMEM106B
• Gene ID (NCBI): 54664
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM106B is a genetic risk factor for frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP). Amyotrophic lateral sclerosis (ALS), like FTLD-TDP, is characterized by pathological TDP-43 inclusions. TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression.

Publications

Species	Application	Title
human	IHC	Cell; Homotypic fibrillization of TMEM106B across diverse neurodegenerative diseases.; Authors - Andrew Chang
human	WB	Brain; C-terminal TMEM106B fragments in human brain correlate with disease-associated TMEM106B haplotypes; Authors - Cristina T Vicente
human	IHC	Acta Neuropathol; Accumulation of TMEM106B C-terminal fragments in neurodegenerative disease and aging; Authors - Jolien Perneel
human	WB	Front Cell Neurosci; Loss of TMEM106B exacerbates C9ALS/FTD DPR pathology by disrupting autophagosome maturation; Authors - Claudia S Bauer ; KD Validated