TMEM119 Polyclonal antibody

TMEM119 Polyclonal Antibody for IHC, IF-P, FC (Intra), ELISA
Cat No. 27585-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, FC (Intra), ELISA

UNQ731/PRO1415, transmembrane protein 119, Osteoblast induction factor, OBIF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 647
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue, mouse liver tissue
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27585-1-AP targets TMEM119 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26269

Product name: Recombinant human TMEM119 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 119-218 aa of NM_181724

Sequence: MRQKQKASAYYPSSFPKKKYVDQSDRAGGPRAFSEVPDRAPDSRPEEALDSSRQLQADILAATQNLKSPTRAALGGGDGARMVEGRGAEEEEKGSQEGDQE

Predict reactive species
Full Name transmembrane protein 119
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberNM_181724
Gene Symbol TMEM119
Gene ID (NCBI) 338773
RRIDAB_2880915
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ4V9L6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788). Microglia can be detected clearly using Catalog#27585-1-AP.

Protocols

Product Specific Protocols
IHC protocol for TMEM119 antibody 27585-1-APDownload protocol
IF protocol for TMEM119 antibody 27585-1-APDownload protocol
FC protocol for TMEM119 antibody 27585-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Sci Adv

Single-cell analysis of human basal cell carcinoma reveals novel regulators of tumor growth and the tumor microenvironment.

Authors - Christian F Guerrero-Juarez
mouse,humanFC

Nat Commun

P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression.

Authors - Eilam Yeini
humanIF

Glia

Transmembrane protein 119 is neither a specific nor a reliable marker for microglia.

Authors - Elise Vankriekelsvenne
mouseIF

Pharmacol Res

MSCs-extracellular vesicles attenuated neuroinflammation, synapse damage and microglial phagocytosis after hypoxia-ischemia injury by preventing osteopontin expression.

Authors - Danqing Xin
mouseIF

Front Immunol

Didymin Suppresses Microglia Pyroptosis and Neuroinflammation Through the Asc/Caspase-1/GSDMD Pathway Following Experimental Intracerebral Hemorrhage.

Authors - Lingui Gu
human

Neurochem Res

BMP7 Attenuates Neuroinflammation after Spinal Cord Injury by Suppressing the Microglia Activation and Inducing Microglial Polarization Via the STAT3 Pathway

Authors - Xiaojin Wei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Marion (Verified Customer) (07-15-2025)

Immunohistofluorescence (PFA-fixed cryosections) image of TMEM119 stained murine brain. Blocking step : 10 % Donkey Serum + 1% BSA + 0.02% Triton as blocking agent for 2 hours at RT Primary antiboby incubation time : 1 night / 4°C Secondary antibody : AlexaFluor 568 (ab175470 ; Abcam) ; 1/200 This antibody does not seem to work under these conditions.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/200 (Also tested at 1/100; 1/400; 1/600 and 1/800)
  • Cell Tissue Type: Mouse Brain
TMEM119 Antibody Immunofluorescence validation (1/200 (Also tested at 1/100; 1/400; 1/600 and 1/800) dilution) in Mouse Brain (Cat no:27585-1-AP)
FH

Hannes (Verified Customer) (07-27-2020)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHC-P image of anti-TMEM119 (27585-1-AP) stained murine and human brain. The tissue was paraffin-embedded, formalin fixed. The tissue was then incubated with EnVision+ Single Reagents (HRP. Rabbit), undiluted for 1 hour at room temperature.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Murine and human brain tissue
TMEM119 Antibody Immunohistochemistry validation (1:500 dilution) in Murine and human brain tissue (Cat no:27585-1-AP)
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