TMEM119 Polyclonal antibody

TMEM119 Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


Human, Mouse and More (2)





Cat no : 27585-1-AP


OBIF, Osteoblast induction factor, TMEM119, transmembrane protein 119, UNQ731/PRO1415

Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHEK-293 cells

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:400-1:1600
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27585-1-AP targets TMEM119 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM119 fusion protein Ag26269
Full Name transmembrane protein 119
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberNM_181724
Gene Symbol TMEM119
Gene ID (NCBI) 338773
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788). Microglia can be detected clearly using Catalog#27585-1-AP.


Product Specific Protocols
IHC protocol for TMEM119 antibody 27585-1-APDownload protocol
FC protocol for TMEM119 antibody 27585-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Sci Adv

Single-cell analysis of human basal cell carcinoma reveals novel regulators of tumor growth and the tumor microenvironment.

Authors - Christian F Guerrero-Juarez

Nat Commun

P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression.

Authors - Eilam Yeini


Transmembrane protein 119 is neither a specific nor a reliable marker for microglia.

Authors - Elise Vankriekelsvenne

Pharmacol Res

MSCs-extracellular vesicles attenuated neuroinflammation, synapse damage and microglial phagocytosis after hypoxia-ischemia injury by preventing osteopontin expression.

Authors - Danqing Xin

Front Immunol

Didymin Suppresses Microglia Pyroptosis and Neuroinflammation Through the Asc/Caspase-1/GSDMD Pathway Following Experimental Intracerebral Hemorrhage.

Authors - Lingui Gu

Neurochem Res

BMP7 Attenuates Neuroinflammation after Spinal Cord Injury by Suppressing the Microglia Activation and Inducing Microglial Polarization Via the STAT3 Pathway

Authors - Xiaojin Wei


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Hannes (Verified Customer) (07-27-2020)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHC-P image of anti-TMEM119 (27585-1-AP) stained murine and human brain. The tissue was paraffin-embedded, formalin fixed. The tissue was then incubated with EnVision+ Single Reagents (HRP. Rabbit), undiluted for 1 hour at room temperature.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Murine and human brain tissue
TMEM119 Antibody Immunohistochemistry validation (1:500 dilution) in Murine and human brain tissue (Cat no:27585-1-AP)