TMEM119 Polyclonal antibody

TMEM119 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27585-1-AP

Synonyms

OBIF, Osteoblast induction factor, TMEM119, transmembrane protein 119, UNQ731/PRO1415



Tested Applications

Positive WB detected inHEK-293 cells, SH-SY5Y cell
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

27585-1-AP targets TMEM119 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, samples.

Tested Reactivity Human, Mouse,
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TMEM119 fusion protein Ag26269
Full Name transmembrane protein 119
Calculated molecular weight 29 kDa
Observed molecular weight 45 kDa
GenBank accession numberNM_181724
Gene symbol TMEM119
Gene ID (NCBI) 338773
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788). Microglia can be detected clearly using Catalog#27585-1-AP. The predicted MW of TMEM119 is 29 kDa. Catalog#27585-1-AP recognizes 45-50 kDa band may due to glycosylation..

Protocols

Product Specific Protocols
WB protocol for TMEM119 antibody 27585-1-APDownload protocol
IHC protocol for TMEM119 antibody 27585-1-APDownload protocol
IF protocol for TMEM119 antibody 27585-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Front Neurol

Early Histone Deacetylase Inhibition Mitigates Ischemia/Reperfusion Brain Injury by Reducing Microglia Activation and Modulating Their Phenotype.

Authors - Shuyuan Li
mouseWB,IF

Exp Cell Res

Toll-like receptor-9 (TLR-9) deficiency alleviates optic nerve injury (ONI) by inhibiting inflammatory response in vivo and in vitro.

Authors - Lu Zhang
mouseIF

Pharmacol Res

MSCs-extracellular vesicles attenuated neuroinflammation, synapse damage and microglial phagocytosis after hypoxia-ischemia injury by preventing osteopontin expression.

Authors - Danqing Xin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hannes (Verified Customer) (07-27-2020)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHC-P image of anti-TMEM119 (27585-1-AP) stained murine and human brain. The tissue was paraffin-embedded, formalin fixed. The tissue was then incubated with EnVision+ Single Reagents (HRP. Rabbit), undiluted for 1 hour at room temperature.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Murine and human brain tissue
TMEM119 Antibody Immunohistochemistry validation (1:500 dilution) in Murine and human brain tissue (Cat no:27585-1-AP)