|Positive WB detected in||HEK-293 cells, SH-SY5Y cell|
|Positive IHC detected in||human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
27585-1-AP targets TMEM119 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, samples.
|Tested Reactivity||Human, Mouse,|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TMEM119 fusion protein Ag26269|
|Full Name||transmembrane protein 119|
|Calculated molecular weight||29 kDa|
|Observed molecular weight||45 kDa|
|GenBank accession number||NM_181724|
|Gene ID (NCBI)||338773|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788). Microglia can be detected clearly using Catalog#27585-1-AP. The predicted MW of TMEM119 is 29 kDa. Catalog#27585-1-AP recognizes 45-50 kDa band may due to glycosylation..
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Hannes (Verified Customer) (07-27-2020)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHC-P image of anti-TMEM119 (27585-1-AP) stained murine and human brain. The tissue was paraffin-embedded, formalin fixed. The tissue was then incubated with EnVision+ Single Reagents (HRP. Rabbit), undiluted for 1 hour at room temperature.