TMEM127 Polyclonal antibody

TMEM127 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 23142-1-AP

Synonyms

Transmembrane protein 127

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Validation Data Gallery

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  IP experiment of HeLa using 23142-1-AP

  IP result of anti-TMEM127 (IP:23142-1-AP, 3ug; Detection:23142-1-AP 1:500) with HeLa cells lysate 3000ug.

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  IHC staining of human stomach cancer using 23142-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23142-1-AP (TMEM127 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 23142-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23142-1-AP (TMEM127 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 23142-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 23142-1-AP (TMEM127 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver cancer using 23142-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 23142-1-AP (TMEM127 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 23142-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 23142-1-AP (TMEM127 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 23142-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TMEM127 antibody (23142-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach cancer tissue, human heart tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

23142-1-AP targets TMEM127 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM127 fusion protein Ag19472
• Full Name: transmembrane protein 127
• Calculated Molecular Weight: 238 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC039892
• Gene Symbol: TMEM127
• Gene ID (NCBI): 55654
• RRID: AB_2879217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	Elife; A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression; Authors - Chaojun Yan
human	WB	Tissue Cell; Exosomes from adipose-derived stem cells overexpressing microRNA-671-3p promote fat graft angiogenesis and adipogenic differentiation; Authors - Xiaoyan Hao