Tested Applications
| Positive IHC detected in | human kidney tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13975-1-AP targets MKS3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5009 Product name: Recombinant human TMEM67 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 33-354 aa of BC032835 Sequence: FQQPEKCDNNQYFDISALSCVPCGANQRQDARGTSCVCLPGFQMISNNGGPAIICKKCPENMKGVTEDGWNCISCPSDLTAEGKCHCPIGHILVERDINGTLLSQATCELCDGNENSFMVVNALGDRCVRCEPTFVNTSRSCACSEPNILTGGLCFSSTGNFPLRRISAARYGEVGMSLTSEWFAKYLQSSAAACWVYANLTSCQALGNMCVMNMNSYDFATFDACGLFQFIFENTAGLSTVHSISFWRQNLPWLFYGDQLGLAPQVLSSTSLPTNFSFKGENQNTKLKFVAASYDIRGNFLKWQTLEGGVLQLCPDTETRL Predict reactive species |
| Full Name | transmembrane protein 67 |
| Calculated Molecular Weight | 112 kDa |
| GenBank Accession Number | BC032835 |
| Gene Symbol | MKS3 |
| Gene ID (NCBI) | 91147 |
| RRID | AB_10638441 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5HYA8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MKS3 protein, also known as Meckelin (encoded by TMEM67 gene), is a transmembrane protein located in primary cilia and plasma membrane. It plays a key role in the migration of centriole to apical membrane and the formation of primary cilia. MKS3 mutation is associated with Meckel syndrome type 3 (MKS3) and Joubert syndrome type 6 (JBTS6).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MKS3 antibody 13975-1-AP | Download protocol |
| IHC protocol for MKS3 antibody 13975-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
MKS3 (TMEM67) polyclonal antibody (Cat# 13975-1-AP) has 46 citations spanning journals including Cell, Nature Cell Biology, Nature Communications, JCI, EMBO Journal, Cell Reports, eLife, and Scientific Reports. The research fields center on ciliogenesis, ciliary transition zone structure, primary cilia assembly, intraflagellar transport, and ciliopathies such as Joubert syndrome and Meckel syndrome.
| Species | Application | Title |
|---|---|---|
Cell The spinocerebellar ataxia-associated gene Tau tubulin kinase 2 controls the initiation of ciliogenesis. | ||
Cell Res CYLD mediates ciliogenesis in multiple organs by deubiquitinating Cep70 and inactivating HDAC6. | ||
Nat Genet A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition. | ||
Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. | ||
Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. | ||
Nat Cell Biol CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base. |
