TMEM70 Polyclonal antibody

• Featured Product
• KD/KO Validated

TMEM70 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 20388-1-AP

Synonyms

TMEM70, transmembrane protein 70

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Validation Data Gallery

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  WB analysis of A2780 using 20388-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 20388-1-AP (TMEM70 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20388-1-AP

  WB result of TMEM70 antibody (20388-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM70 transfected Jurkat cells.

• 

  WB analysis of A2780 using 20388-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 20388-1-AP (TMEM70 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 20388-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20388-1-AP (TMEM70 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 20388-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20388-1-AP (TMEM70 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A2780 cells, Jurkat cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

20388-1-AP targets TMEM70 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM70 fusion protein Ag14226
• Full Name: transmembrane protein 70
• Calculated Molecular Weight: 260 aa, 29 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC002748
• Gene Symbol: TMEM70
• Gene ID (NCBI): 54968
• RRID: AB_10694436
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM70 belongs to the TMEM70 family. It is involved in biogenesis of mitochondrial ATP synthase. Defects in TMEM70 are a cause of mitochondrial encephalocardiomyopathy neonatal due to ATP synthase deficiency (MT-ATPSD). A publication(PMID:21147908) identifies that TMEM70 gene defect as a pan-ethnic disorder and further redefines it as the most common cause of nuclear-origin ATP synthase deficiency.

Publications

Species	Application	Title
human	WB	Mitochondrion; Mitochondrial membrane assembly of TMEM70 protein.; Authors - Hana Kratochvílová
human	WB	Neurogenetics; TMEM70: a mutational hot spot in nuclear ATP synthase deficiency with a pivotal role in complex V biogenesis.; Authors - Torraco Alessandra A
human	WB	JIMD Rep; Common and Novel TMEM70 Mutations in a Cohort of Italian Patients with Mitochondrial Encephalocardiomyopathy.; Authors - Daria Diodato
human	WB	Life Sci Alliance; Mitochondrial stress response triggered by defects in protein synthesis quality control.; Authors - Uwe Richter ; KD Validated