TMF1-Specific antibody (19728-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 19728-1-AP (same clone as 19728-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 19728-1-AP (TMF1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.
WB analysis of BxPC-3 using 19728-1-AP (same clone as 19728-1-PBS)
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 19728-1-AP (TMF1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.
WB analysis of L02 using 19728-1-AP (same clone as 19728-1-PBS)
L02 cells were subjected to SDS PAGE followed by western blot with 19728-1-AP (TMF1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.
IP experiment of HepG2 using 19728-1-AP (same clone as 19728-1-PBS)
IP result of anti-TMF1-Specific (IP:19728-1-AP, 4ug; Detection:19728-1-AP 1:300) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 19728-1-AP (same clone as 19728-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 19728-1-AP (same clone as 19728-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 19728-1-AP (same clone as 19728-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 19728-1-AP (same clone as 19728-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 19728-1-AP (same clone as 19728-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 19728-1-AP (same clone as 19728-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 19728-1-AP (same clone as 19728-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 19728-1-AP (TMF1-Specific antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19728-1-PBS in a different storage buffer formulation.

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Tested Applications
Product Information

Product Information

19728-1-PBS targets TMF1-Specific in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	TATA element modulatory factor 1
Calculated Molecular Weight	123 kDa
Observed Molecular Weight	150-160 kDa
GenBank Accession Number	NM_007114
Gene Symbol	TMF1
Gene ID (NCBI)	7110
RRID	AB_10667009
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P82094
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

TMF1, also named as ARA160, is a potential coactivator of the androgen receptor. TMF1 mediates STAT3 degradation. TMF1 plays critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. TMF1 binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). The antibody is specific to TMF1. TMF1 can be detected as 150-160 kDa after posttranslational modification.