|Positive WB detected in
|THP-1 cells, LPS treated RAW 264.7 cells
|Western Blot (WB)
|WB : 1:500-1:2000
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
17590-1-AP targets TNF Alpha in WB, Transwell Assay, Cell treatment, ELISA applications and shows reactivity with human, mouse samples.
|human, mouse, rat, rabbit, fish, bovine
|Host / Isotype
|Rabbit / IgG
|TNF Alpha fusion protein Ag11433
|tumor necrosis factor (TNF superfamily, member 2)
|Calculated molecular weight
|233 aa, 26 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Margarita (Verified Customer) (11-22-2023)
Prostate Cancer cell line, overnight incubation, Mouse
Isha (Verified Customer) (09-20-2021)
signal was very good. My sample was renal tissue lysate after ischemia reperfusion