TNF Alpha Polyclonal antibody
TNF Alpha Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (3)
Applications
WB, Transwell Assay, Cell treatment, ELISA
Conjugate
Unconjugated
398
Cat no : 17590-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | THP-1 cells, LPS treated RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 328 publications below |
| IHC | See 57 publications below |
| IF | See 18 publications below |
| ELISA | See 3 publications below |
Product Information
17590-1-AP targets TNF Alpha in WB, Transwell Assay, Cell treatment, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, fish, rabbit |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TNF Alpha fusion protein Ag11433 |
| Full Name | tumor necrosis factor (TNF superfamily, member 2) |
| Calculated molecular weight | 233 aa, 26 kDa |
| Observed molecular weight | 26 kDa |
| GenBank accession number | BC028148 |
| Gene symbol | TNF |
| Gene ID (NCBI) | 7124 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TNF Alpha antibody 17590-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Redox Biol Icariin alleviates uveitis by targeting peroxiredoxin 3 to modulate retinal microglia M1/M2 phenotypic polarization. | ||
J Immunother Cancer Rationalized inhibition of mixed lineage kinase 3 and CD70 enhances life span and antitumor efficacy of CD8+ T cells. | ||
Adv Sci (Weinh) A Ultrasensitive Near-Infrared Fluorescent Probe Reveals Pyroglutamate Aminopeptidase 1 Can Be a New Inflammatory Cytokine. | ||
ACS Appl Mater Interfaces 3D-Printed Silk Sericin-Based Hydrogel Scaffold：A Promising Visualized Dressing Material for Real-Time Monitoring of Wounds. | ||
Bone Res Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines. | ||
Oxid Med Cell Longev HBO1 as an Important Target for the Treatment of CCL4-Induced Liver Fibrosis and Aged-Related Liver Aging and Fibrosis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Isha (Verified Customer) (09-20-2021) | signal was very good. My sample was renal tissue lysate after ischemia reperfusion
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