TNF-alpha Polyclonal antibody

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  WB analysis using 17590-1-AP

  Untreated THP-1 and THP-1 (treated with 80nM PMA overnight, replaced the culture medium with 100 ng/ml LPS for 6 hours with addition of 300ng/ml Brefeldin A for the last 3 hours) cells were subjected to SDS PAGE followed by western blot with 17590-1-AP (TNF Alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17590-1-AP

  Untreated THP-1 and THP-1 (treated with 80nM PMA overnight, replaced the culture medium with 100 ng/ml LPS for 6 hours with addition of 300ng/ml Brefeldin A for the last 3 hours) cells were subjected to SDS PAGE followed by western blot with 17590-1-AP (TNF-alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 17590-1-AP

  LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 17590-1-AP (TNF alpha antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 17590-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PMA, LPS and Brefeldin A treated THP-1 cells using TNF-alpha antibody (17590-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TNF-alpha Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 17590-1-AP

Publications(769)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (5)

Applications

WB, IHC, IF/ICC, ELISA, Cell treatment, Transwell Assay

Synonyms

TNF, TNF alpha, TNF-a, C-domain 1, C-domain 2

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Tested Applications

• Positive WB detected in: THP-1 cells, LPS treated RAW 264.7 cells
• Positive IF/ICC detected in: PMA, LPS and Brefeldin A treated THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 615 publications below
• IHC: See 120 publications below
• IF: See 63 publications below
• ELISA: See 3 publications below

Product Information

17590-1-AP targets TNF-alpha in WB, IHC, IF/ICC, ELISA, Cell treatment, Transwell Assay applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, rabbit, bovine, sheep, fish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNF-alpha fusion protein Ag11433
• Full Name: tumor necrosis factor (TNF superfamily, member 2)
• Calculated Molecular Weight: 233 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC028148
• Gene Symbol: TNF-alpha
• Gene ID (NCBI): 7124
• ENSEMBL Gene ID: ENSG00000232810
• RRID: AB_2271853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P01375
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.

Publications

Species	Application	Title
mouse	WB	Brain Behav Immun; HMGB1 mediates depressive behavior induced by chronic stress through activating the kynurenine pathway.; Authors - Bo Wang
mouse	IHC	Bioact Mater; A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds; Authors - Shunlai Shang
mouse	WB,IHC,IF	ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang
mouse	WB	Adv Sci (Weinh); 3D Printing of a Vascularized Mini-Liver Based on the Size-Dependent Functional Enhancements of Cell Spheroids for Rescue of Liver Failure; Authors - Jiabin Zhang
mouse	WB	Sci Adv; Citrulline regulates macrophage metabolism and inflammation to counter aging in mice; Authors - Zhangdan Xie
	WB,IF	Small; M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation; Authors - Jie Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Margarita (Verified Customer) (11-22-2023)

Prostate Cancer cell line, overnight incubation, Mouse

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: TRAMPC C2

TNF-alpha Antibody Immunofluorescence validation (1:100 dilution) in TRAMPC C2 (Cat no:17590-1-AP)

FH

Isha (Verified Customer) (09-20-2021)

signal was very good. My sample was renal tissue lysate after ischemia reperfusion

• Applications: Western Blot
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: Kidney