|Positive WB detected in||THP-1 cells, LPS treated RAW 264.7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
17590-1-AP targets TNF Alpha in WB, Transwell Assay, Cell treatment, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, fish, rabbit|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TNF Alpha fusion protein Ag11433|
|Full Name||tumor necrosis factor (TNF superfamily, member 2)|
|Calculated molecular weight||233 aa, 26 kDa|
|Observed molecular weight||26 kDa|
|GenBank accession number||BC028148|
|Gene ID (NCBI)||7124|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.
Icariin alleviates uveitis by targeting peroxiredoxin 3 to modulate retinal microglia M1/M2 phenotypic polarization.
J Immunother Cancer
Rationalized inhibition of mixed lineage kinase 3 and CD70 enhances life span and antitumor efficacy of CD8+ T cells.
Adv Sci (Weinh)
A Ultrasensitive Near-Infrared Fluorescent Probe Reveals Pyroglutamate Aminopeptidase 1 Can Be a New Inflammatory Cytokine.
ACS Appl Mater Interfaces
3D-Printed Silk Sericin-Based Hydrogel Scaffold：A Promising Visualized Dressing Material for Real-Time Monitoring of Wounds.
Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines.
Oxid Med Cell Longev
HBO1 as an Important Target for the Treatment of CCL4-Induced Liver Fibrosis and Aged-Related Liver Aging and Fibrosis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Isha (Verified Customer) (09-20-2021)
signal was very good. My sample was renal tissue lysate after ischemia reperfusion