DR5-Specific Polyclonal antibody

DR5-Specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15497-1-AP

Synonyms

CD262, Death receptor 5, DR5, KILLER, KILLER/DR5, TNFRSF10B, TRAIL R2, TRAIL receptor 2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9



Tested Applications

Positive WB detected inHL-60 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

15497-1-AP targets DR5-Specific in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name tumor necrosis factor receptor superfamily, member 10b
Calculated molecular weight 48 kDa
Observed molecular weight 58-60 kDa, 45-50 kDa, 32 kDa
GenBank accession numberNM_003842
Gene symbol TNFRSF10B
Gene ID (NCBI) 8795
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TNFRSF10B, also known as CD262, DR5, TRAILR2, TRICK2 and KILLER, is a widely expressed single-pass type I membrane protein belonging to the tumour necrosis factor receptor superfamily (TNFRSF). It is a receptor for TNF-related apoptosis-inducing ligand (TRAIL), which is a member of the tumor necrosis factor (TNF) family of cytokines and induces apoptosis in a wide variety of cells (PMID: 9311998). TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain (DD), through which TNFRSF10B is capable to transmit the apoptotic signal (PMID: 9311998; 20531300). In PDAC cells, especially Panc89 cells, it expresses additionally to the approximately 48 and 40 kDa forms of DR5, a faster migrating variant of DR5 of about 32 kDa(PMID:20354842). The TRAIL receptor 2 (death receptor 5, or DR5) forms receptor dimers in a ligand-dependent manner, and these receptor dimers exist within high molecular weight networks(Dr. Jonathan N. Sachs,2012).

Protocols

Product Specific Protocols
WB protocol for DR5-Specific antibody 15497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Biochem Biophys Res Commun

CRISPR-mediated upregulation of DR5 and downregulation of cFLIP synergistically sensitize HeLa cells to TRAIL-mediated apoptosis.

Authors - Naresh Poondla
humanWB

Acta Biochim Biophys Sin (Shanghai)

Trail armed oncolytic poxvirus suppresses lung cancer cell by inducing apoptosis.

Authors - Jinqing Hu
humanWB

Oncol Lett

YM155 sensitizes HeLa cells to TRAIL-mediated apoptosis via cFLIP and survivin downregulation.

Authors - Arun Pandian Chandrasekaran
humanWB

Arch Biochem Biophys

Luteolin enhances TRAIL sensitivity in non-small cell lung cancer cells through increasing DR5 expression and Drp1-mediated mitochondrial fission.

Authors - Bin Wu
humanWB

Mol Pharm

Discovery and characterization of 4-hydroxy-2-pyridone derivative sambutoxin as a potent and promising anticancer drug candidate: activity and molecular mechanism.

Authors - Lu-Ning Li
humanWB

Nat Commun

A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure.

Authors - Yufeng Yao