DR5-Specific Polyclonal antibody

• 

  WB analysis of PC-3 cells using 15497-1-AP

  WB result of 15497-1-AP (TNFRSF10B, DR5 antibody) with PC-3 cell, untreated or treated with tunicamysin.

• 

  WB analysis of HL-60 using 15497-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15497-1-AP (TNFRSF10B-Specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

DR5-Specific Polyclonal Antibody for WB, ELISA

Cat No. 15497-1-AP

Publications(15)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CD262, Death receptor 5, DR5, KILLER, KILLER/DR5, TNFRSF10B, TRAIL R2, TRAIL receptor 2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9

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Tested Applications

• Positive WB detected in: HL-60 cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below

Product Information

15497-1-AP targets DR5-Specific in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tumor necrosis factor receptor superfamily, member 10b
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 58-60 kDa, 45-50 kDa, 32 kDa
• GenBank Accession Number: NM_003842
• Gene Symbol: DR5
• Gene ID (NCBI): 8795
• RRID: AB_2240702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14763
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNFRSF10B, also known as CD262, DR5, TRAILR2, TRICK2 and KILLER, is a widely expressed single-pass type I membrane protein belonging to the tumour necrosis factor receptor superfamily (TNFRSF). It is a receptor for TNF-related apoptosis-inducing ligand (TRAIL), which is a member of the tumor necrosis factor (TNF) family of cytokines and induces apoptosis in a wide variety of cells (PMID: 9311998). TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain (DD), through which TNFRSF10B is capable to transmit the apoptotic signal (PMID: 9311998; 20531300). In PDAC cells, especially Panc89 cells, it expresses additionally to the approximately 48 and 40 kDa forms of DR5, a faster migrating variant of DR5 of about 32 kDa(PMID:20354842). The TRAIL receptor 2 (death receptor 5, or DR5) forms receptor dimers in a ligand-dependent manner, and these receptor dimers exist within high molecular weight networks(Dr. Jonathan N. Sachs,2012).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure.; Authors - Yufeng Yao
human	WB	Int J Biol Sci; ESRG is critical to maintain the cell survival and self-renewal/pluripotency of hPSCs by collaborating with MCM2 to suppress p53 pathway; Authors - Shasha Li
human	WB	Biomed Pharmacother; Pro-survival effects by NF-κB, Akt and ERK(1/2) and anti-apoptosis actions by Six1 disrupt apoptotic functions of TRAIL-Dr4/5 pathway in ovarian cancer.; Authors - Juan Yang
human	WB	iScience; A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism.; Authors - Muzammal Hussain
human	WB	Mol Pharm; Discovery and characterization of 4-hydroxy-2-pyridone derivative sambutoxin as a potent and promising anticancer drug candidate: activity and molecular mechanism.; Authors - Lu-Ning Li
human	WB	Arch Biochem Biophys; Luteolin enhances TRAIL sensitivity in non-small cell lung cancer cells through increasing DR5 expression and Drp1-mediated mitochondrial fission.; Authors - Bin Wu