BCMA/TNFRSF17 Polyclonal antibody, PBS Only

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  WB analysis of Daudi using 27724-1-AP (same clone as 27724-1-PBS)

  Daudi and Ramos cells were subjected to SDS PAGE followed by western blot with 27724-1-AP (TNFRSF17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27724-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 27724-1-AP (same clone as 27724-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 27724-1-AP (TNFRSF17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27724-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Ramos using 27724-1-AP (same clone as 27724-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 27724-1-AP (TNFRSF17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27724-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 27724-1-AP (same clone as 27724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27724-1-AP (TNFRSF17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27724-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 27724-1-AP (same clone as 27724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27724-1-AP (TNFRSF17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27724-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 27724-1-AP (same clone as 27724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27724-1-AP (TNFRSF17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27724-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 27724-1-AP (same clone as 27724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27724-1-AP (TNFRSF17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27724-1-PBS in a different storage buffer formulation.

BCMA/TNFRSF17 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 27724-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

TNFRSF17, CD269, BCMA, BCM, B-cell maturation protein

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Product Information

27724-1-PBS targets BCMA/TNFRSF17 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27049

  Product name: Recombinant human TNFRSF17 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 78-184 aa of BC058291

  Sequence: RKINSEPLKDEFKNTGSGLLGMANIDLEKSRTGDEIILPRGLEYTVEECTCEDCIKSKPKVDSDHCFPLPAMEEGATILVTTKTNDYCKSLPAALSATEIEKSISAR

• Full Name: tumor necrosis factor receptor superfamily, member 17
• Calculated Molecular Weight: 184 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC058291
• Gene Symbol: TNFRSF17
• Gene ID (NCBI): 608
• RRID: AB_2880954
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q02223
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BCMA (B cell maturation antigen), also known as TNFRSF17, is 20.2-kDa type III transmembrane glycoprotein and is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes and plasma cells, which may be important for B cell development and autoimmune response (PMID: 32943087; 9846698). BCMA has two agonist ligands: a proliferation-inducing ligand (APRIL) and BAFF. When BCMA binds to APRIL, it transmits signals of cell survival and proliferation (PMID: 27127303); when BCMA binds to BAFF, it mediates the activation of NF-kappaB and MAPK8/JNK (PMID: 36140254). It has been found that the overexpression and activation of BCMA are associated with multiple myeloma (MM) in preclinical models and humans, supporting its potential utility as a therapeutic target for MM (PMID: 32055000; 32943087).