Tenascin-X Polyclonal antibody

Tenascin-X Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 13595-1-AP

Synonyms

Hexabrachion like protein, HXBL, Tenascin X, tenascin XB, Tenascin-X, TENX, TN X, TNX, TNXB, TNXB1, TNXB2, TNXBS, XB, XBS

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Validation Data Gallery

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  WB analysis of mouse liver using 13595-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13595-1-AP (Tenascin-X antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 13595-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13595-1-AP (Tenascin-X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 13595-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 13595-1-AP (Tenascin-X antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 13595-1-AP

  IP result of anti-Tenascin-X (IP:13595-1-AP, 4ug; Detection:13595-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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  IHC staining of human kidney using 13595-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 13595-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 13595-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 13595-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 13595-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HT-1080 cells, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human kidney tissue, human heart tissue, human lung tissue, human breast cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

13595-1-AP targets Tenascin-X in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Tenascin-X fusion protein Ag4501
• Full Name: tenascin XB
• Calculated Molecular Weight: 4289 aa, 464 kDa
• Observed Molecular Weight: 75 kDa, 140 kDa
• GenBank Accession Number: BC033740
• Gene Symbol: TNXB
• Gene ID (NCBI): 7148
• RRID: AB_10644124
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tenascin-X (TNXB) is an extracellular matrix glycoprotein predominantly located in the outer reticular lamina of the basement membrane (BM) (PMID: 23768946). It interacts with many other ECM proteins and it accelerates collagen fibrillogenesis in vitro. TNXB plays a role in interactions between cell and ECM, antiadhesive effect, inhibiting cell migration, and in maintaining homeostasis of the extracellular matrix. Deficiency of TNXB has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This antibody is raised against human TNXB and detects a band of about 75 kDa, which probably represents a proteolytic cleaved form of human TNXB (PMID: 17263730). But the serum form of tenascin-X is a 140-kD protein probably resulting from proteolytic cleavage or alternative splicing of tenascin-X(PMID: 16567571, 11642233).

Publications

Species	Application	Title
human	WB,IHC,IF	J Am Soc Nephrol; TNXB mutations can cause vesicoureteral reflux.; Authors - Rasheed A Gbadegesin
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	WB,IHC	World J Gastroenterol; Role of Tenascin-X in regulating TGF-β/Smad signaling pathway in pathogenesis of slow transit constipation.; Authors - Yi-Chao Zhang
human	WB,IHC	Mol Oncol; Integrated proteome and phosphoproteome analysis of gastric adenocarcinoma reveals molecular signatures capable of stratifying patient outcome; Authors - Xue Lu
mouse	WB,IF	Arterioscler Thromb Vasc Biol; Loss of Smooth Muscle Tenascin-X Inhibits Vascular Remodeling Through Increased TGF-β Signaling; Authors - Guozheng Liang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iram (Verified Customer) (09-04-2020)

Very sharp bands of TenascinX in western blotting

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 for western
• Cell Tissue Type: Human colon cancer