TOM1L1 Polyclonal antibody

• 

  WB analysis using 51017-1-AP

  BT-474 cells were subjected to SDS PAGE followed by western blot with 51017-1-AP (TOM1L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 51017-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 51017-1-AP (TOM1L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

TOM1L1 Polyclonal Antibody for WB, ELISA

Cat No. 51017-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

Src-activating and signaling molecule protein, Target of Myb-like protein 1, TOM1 L1, TOM1 like protein 1, TOM1L1 protein

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Tested Applications

• Positive WB detected in: BT-474 cells, SGC-7901 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below

Product Information

51017-1-AP targets TOM1L1 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0502

  Product name: Recombinant human TOM1L1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 381-1/1 of BC029396

  Sequence:

• Full Name: target of myb1 (chicken)-like 1
• Calculated Molecular Weight: 476 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC029396
• Gene Symbol: TOM1L1
• Gene ID (NCBI): 10040
• RRID: AB_2207419
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75674
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Target of Myb-like protein 1 (TOM1L1), also known as Srcasm, belongs to the Tom family. TOM1L1 encodes an adaptor protein characterized by an N-terminal VHS domain and a central GAT domain, which facilitates interactions with clathrin and components of the endosomal sorting machinery. Functionally, TOM1L1 serves as a substrate for Src family kinases, playing a regulatory role in growth factor signaling and membrane trafficking. It is involved in endocytosis, signal transduction, and the negative regulation of mitotic cell division. (PMID: 17977829, PMID: 26899482, PMID: 35238864, PMID: 39548458)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP	Exp Cell Res; Mechanisms underlying melanoma invasion as a consequence of MLK3 loss; Authors - Henriette U Balinda