TOMM34 Polyclonal antibody

TOMM34 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12196-1-AP

Synonyms

hTom34, HTOM34P, TOM34, TOMM34, URCC3

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Validation Data Gallery

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  WB analysis using 12196-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12196-1-AP (TOMM34 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12196-1-AP

  IP Result of anti-TOMM34 (IP:12196-1-AP, 4ug; Detection:12196-1-AP 1:300) with HeLa cells lysate 4000ug.

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  IHC staining of human colon cancer using 12196-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12196-1-AP (TOMM34 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of MCF-7 using 12196-1-AP

  Immunofluorescent analysis of MCF-7 cells, using TOMM34 antibody 12196-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: A375 cells, HeLa cells, PC-3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

12196-1-AP targets TOMM34 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TOMM34 fusion protein Ag2834
• Full Name: translocase of outer mitochondrial membrane 34
• Calculated molecular weight: 309 aa, 35 kDa
• Observed molecular weight: 31-35 kDa
• GenBank accession number: BC014907
• Gene symbol: TOMM34
• Gene ID (NCBI): 10953
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; Proteomic analysis of the glutathione-deficient LEGSKO mouse lens reveals activation of EMT signaling, loss of lens specific markers, and changes in stress response proteins.; Authors - Jeremy A Whitson
human	IHC	Pathol Oncol Res; High Expression of Tomm34 and Its Correlations With Clinicopathology in Oral Squamous Cell Carcinoma.; Authors - Min Cai
	IHC	Acta Neuropathol; The proteome of granulovacuolar degeneration and neurofibrillary tangles in Alzheimer's disease.; Authors - David C Hondius
human	WB	J Oral Pathol Med; TOMM34 promotes cell proliferation, migration and invasion of OSCC and modulates mitochondrial function; Authors - Yunyi Huang ; KD Validated