TOMM34 Polyclonal antibody

TOMM34 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12196-1-AP

Synonyms

hTom34, HTOM34P, TOM34, TOMM34, URCC3



Tested Applications

Positive WB detected inA375 cells, HeLa cells, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12196-1-AP targets TOMM34 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOMM34 fusion protein Ag2834
Full Name translocase of outer mitochondrial membrane 34
Calculated molecular weight 309 aa, 35 kDa
Observed molecular weight 31-35 kDa
GenBank accession numberBC014907
Gene symbol TOMM34
Gene ID (NCBI) 10953
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TOMM34 antibody 12196-1-APDownload protocol
IHC protocol for TOMM34 antibody 12196-1-APDownload protocol
IF protocol for TOMM34 antibody 12196-1-APDownload protocol
IP protocol for TOMM34 antibody 12196-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Pathol Oncol Res

High Expression of Tomm34 and Its Correlations With Clinicopathology in Oral Squamous Cell Carcinoma.

Authors - Min Cai
mouseWB

Free Radic Biol Med

Proteomic analysis of the glutathione-deficient LEGSKO mouse lens reveals activation of EMT signaling, loss of lens specific markers, and changes in stress response proteins.

Authors - Jeremy A Whitson