TP53BP1-Specific Polyclonal antibody, PBS Only

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  WB analysis using 20002-1-AP (same clone as 20002-1-PBS)

  HeLa lysates were subjected to SDS PAGE followed by western blot with 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20002-1-AP (same clone as 20002-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 20002-1-AP (same clone as 20002-1-PBS)

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 20002-1-AP (same clone as 20002-1-PBS)

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20002-1-AP (same clone as 20002-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TP53BP1-Specific antibody (20002-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

TP53BP1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 20002-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

TP53BP1, 53BP1, FLJ41424, p202, p53 binding protein 1

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Product Information

20002-1-PBS targets TP53BP1-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tumor protein p53 binding protein 1
• Calculated Molecular Weight: 214 kDa
• Observed Molecular Weight: 450 kDa
• GenBank Accession Number: NM_005657
• Gene Symbol: TP53BP1
• Gene ID (NCBI): 7158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12888
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TP53BP1, also named as p53BP1, p202, 53BP1 and FLJ41424, may have a role in checkpoint signaling during mitosis. TP53BP1 enhances TP53-mediated transcriptional activation. TP53BP1 plays a role in the response to DNA damage. This antibody is specific to TP53BP1.