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  • KD/KO Validated

TP53RK Polyclonal antibody

TP53RK Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 26818-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

BUD32, C20orf64, dJ101A2, Nori 2, Nori 2p, p53 related protein kinase, PRPK, TP53 regulating kinase, TP53RK

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inJurkat cells, HepG2 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26818-1-AP targets TP53RK in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25326

Product name: Recombinant human TP53RK protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 154-253 aa of BC009727

Sequence: HDEDLIHGDLTTSNMLLKPPLEQLNIVLIDFGLSFISALPEDKGVDLYVLEKAFLSTHPNTETVFEAFLKSYSTSSKKARPVLKKLDEVRLRGRKRSMVG

Predict reactive species
Full Name TP53 regulating kinase
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC009727
Gene Symbol TP53RK
Gene ID (NCBI) 112858
RRIDAB_2880647
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96S44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TP53RK antibody 26818-1-APDownload protocol
IHC protocol for TP53RK antibody 26818-1-APDownload protocol
IP protocol for TP53RK antibody 26818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Biol Med

Systematic screening for potential therapeutic targets in osteosarcoma through a kinome-wide CRISPR-Cas9 library.

Authors - Yuanzhong Wu
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Evangelia (Verified Customer) (10-05-2022)

It could be used in higher dilution. The 1/1000 works really strong.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: -
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