TRAF1 Polyclonal antibody

TRAF1 Polyclonal Antibody for FC, IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 26845-1-AP

Synonyms

EBI6, TRAF1



Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26845-1-AP is TRAF1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAF1 fusion protein Ag25397
Full Name TNF receptor-associated factor 1
Calculated Molecular Weight 416 aa, 46 kDa
GenBank Accession NumberBC024145
Gene Symbol TRAF1
Gene ID (NCBI) 7185
RRIDAB_2880655
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for TRAF1 antibody 26845-1-APDownload protocol
IF protocol for TRAF1 antibody 26845-1-APDownload protocol
FC protocol for TRAF1 antibody 26845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Lett

Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.

Authors - Bei Wang
ratWB

J Cell Mol Med

Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.

Authors - Yong Zhang
humanWB

Front Pharmacol

Anthraquinone Emodin Inhibits Tumor Necrosis Factor Alpha-Induced Calcification of Human Aortic Valve Interstitial Cells via the NF-κB Pathway.

Authors - Kang Xu
humanWB

Int Immunopharmacol

Autoinducer-2 of Fusobacterium nucleatum promotes macrophage M1 polarization via TNFSF9/IL-1β signaling.

Authors - Jiao Wu
humanWB

Int Urol Nephrol

TRAIP suppressed apoptosis and cell cycle to promote prostate cancer proliferation via TRAF2-PI3K-AKT pathway activation

Authors - Yuan Gu
humanWB,IHC

J Exp Clin Cancer Res

DNMT1-induced miR-378a-3p silencing promotes angiogenesis via the NF-κB signaling pathway by targeting TRAF1 in hepatocellular carcinoma

Authors - Bin Zhu