TRAF1 Polyclonal antibody

TRAF1 Polyclonal Antibody for FC, IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC

Conjugate

Unconjugated

Publications

5

Cat no : 26845-1-AP

Synonyms

EBI6, TRAF1

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Validation Data Gallery

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  IHC staining of human tonsillitis using 26845-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26845-1-AP (TRAF1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human tonsillitis using 26845-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26845-1-AP (TRAF1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human spleen using 26845-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 26845-1-AP (TRAF1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 26845-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 26845-1-AP (TRAF1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HUVEC using 26845-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HUVEC cells using 26845-1-AP (TRAF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Raji using 26845-1-AP

  1X10^6 Raji cells were intracellularly stained with 0.20 ug/test Anti-Human TRAF1 (26845-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with 90% MeOH.

• 

  FC experiment of Raji using 26845-1-AP

  1X10^6 Raji cells were surface stained with 0.5 ug Anti-Human TRAF1 (26845-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HUVEC cells
• Positive FC detected in: Raji cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 26845-1-AP is TRAF1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAF1 fusion protein Ag25397
• Full Name: TNF receptor-associated factor 1
• Calculated molecular weight: 416 aa, 46 kDa
• GenBank accession number: BC024145
• Gene symbol: TRAF1
• Gene ID (NCBI): 7185
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cancer Lett; Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.; Authors - Bei Wang
rat	WB	J Cell Mol Med; Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.; Authors - Yong Zhang
human	WB	Front Pharmacol; Anthraquinone Emodin Inhibits Tumor Necrosis Factor Alpha-Induced Calcification of Human Aortic Valve Interstitial Cells via the NF-κB Pathway.; Authors - Kang Xu
human	WB	Int Immunopharmacol; Autoinducer-2 of Fusobacterium nucleatum promotes macrophage M1 polarization via TNFSF9/IL-1β signaling.; Authors - Jiao Wu
human	WB,IHC	J Exp Clin Cancer Res; DNMT1-induced miR-378a-3p silencing promotes angiogenesis via the NF-κB signaling pathway by targeting TRAF1 in hepatocellular carcinoma; Authors - Bin Zhu