TRAIL/CD253 Polyclonal antibody, PBS Only

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  WB analysis using 27064-1-AP (same clone as 27064-1-PBS)

  Human milk sample were subjected to SDS PAGE followed by western blot with 27064-1-AP (TRAIL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 27064-1-AP (same clone as 27064-1-PBS)

  Human milk were subjected to SDS PAGE followed by western blot with 27064-1-AP (TRAIL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 27064-1-AP (same clone as 27064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 27064-1-AP (same clone as 27064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 27064-1-AP (same clone as 27064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27064-1-AP (TRAIL/CD253 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 27064-1-AP (same clone as 27064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27064-1-AP (TRAIL/CD253 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 27064-1-AP (same clone as 27064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 27064-1-AP (same clone as 27064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 27064-1-AP (same clone as 27064-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 27064-1-AP (TRAIL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

TRAIL/CD253 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 27064-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TNFSF10, TRAIL, Apo-2 ligand, CD253, Protein TRAIL

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Product Information

27064-1-PBS targets TRAIL/CD253 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25746

  Product name: Recombinant human TRAIL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 39-281 aa of BC032722

  Sequence: TNELKQMQDKYSKSGIACFLKEDDSYWDPNDEESMNSPCWQVKWQLRQLVRKMILRTSEETISTVQEKQQNISPLVRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG

• Full Name: tumor necrosis factor (ligand) superfamily, member 10
• Calculated Molecular Weight: 281 aa, 33 kDa
• Observed Molecular Weight: 33 kDa, 20 kDa
• GenBank Accession Number: BC032722
• Gene Symbol: TRAIL
• Gene ID (NCBI): 8743
• RRID: AB_2880737
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P50591
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.