TRAIL Polyclonal antibody

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  WB analysis using 27064-1-AP

  Human milk sample were subjected to SDS PAGE followed by western blot with 27064-1-AP (TRAIL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 27064-1-AP

  Human milk were subjected to SDS PAGE followed by western blot with 27064-1-AP (TRAIL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 27064-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 27064-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 27064-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 27064-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 27064-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 27064-1-AP (TRAIL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

TRAIL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 27064-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TNFSF10, TNFSF 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, CD253

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Tested Applications

• Positive WB detected in: human milk
• Positive IHC detected in: human kidney tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

27064-1-AP targets TRAIL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRAIL fusion protein Ag25746
• Full Name: tumor necrosis factor (ligand) superfamily, member 10
• Calculated Molecular Weight: 281 aa, 33 kDa
• Observed Molecular Weight: 33 kDa, 20 kDa
• GenBank Accession Number: BC032722
• Gene Symbol: TRAIL
• Gene ID (NCBI): 8743
• RRID: AB_2880737
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P50591
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.

Publications

Species	Application	Title
human	IF	Stem Cells Transl Med; Site-Specific Integration of TRAIL in iPSC-Derived Mesenchymal Stem Cells for Targeted Cancer Therapy.; Authors - Zujia Wang
human	WB	Int J Pharm; Fusion of an EGFR-antagonistic affibody enhances the anti-tumor effect of TRAIL to EGFR positive tumors.; Authors - Dianlong Jia
human	WB	Arthritis Res Ther; IL13Rα1 protects against rheumatoid arthritis by combating the apoptotic resistance of fibroblast-like synoviocytes.; Authors - Xiaomei Yang
human	WB	Cell Biosci; Long noncoding RNA TANCR promotes γδ T cells activation by regulating TRAIL expression in cis.; Authors - Chuan Yang ; KD Validated
human	WB	J Nat Prod; Sanggenon C Suppresses Tumorigenesis of Gastric Cancer by Blocking ERK-Drp1-Mediated Mitochondrial Fission; Authors - Xiao-Jie Chen
human	WB	Phytomedicine; Evodiamine inhibits ESCC by inducing M-phase cell-cycle arrest via CUL4A/p53/p21 axis and activating noxa-dependent intrinsic and DR4-dependent extrinsic apoptosis; Authors - Li Zhang