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- KD/KO Validated
TRAIL Polyclonal antibody
TRAIL Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 27064-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human milk |
Positive IHC detected in | human kidney tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 5 publications below |
IF | See 1 publications below |
Product Information
The immunogen of 27064-1-AP is TRAIL Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TRAIL fusion protein Ag25746 |
Full Name | tumor necrosis factor (ligand) superfamily, member 10 |
Calculated molecular weight | 281 aa, 33 kDa |
Observed molecular weight | 33 kDa, 20 kDa |
GenBank accession number | BC032722 |
Gene symbol | TNFSF10 |
Gene ID (NCBI) | 8743 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for TRAIL antibody 27064-1-AP | Download protocol |
IHC protocol for TRAIL antibody 27064-1-AP | Download protocol |
IF protocol for TRAIL antibody 27064-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Stem Cells Transl Med Site-Specific Integration of TRAIL in iPSC-Derived Mesenchymal Stem Cells for Targeted Cancer Therapy. | ||
Int J Pharm Fusion of an EGFR-antagonistic affibody enhances the anti-tumor effect of TRAIL to EGFR positive tumors. | ||
Arthritis Res Ther IL13Rα1 protects against rheumatoid arthritis by combating the apoptotic resistance of fibroblast-like synoviocytes. | ||
Cell Biosci Long noncoding RNA TANCR promotes γδ T cells activation by regulating TRAIL expression in cis.
| ||
J Nat Prod Sanggenon C Suppresses Tumorigenesis of Gastric Cancer by Blocking ERK-Drp1-Mediated Mitochondrial Fission | ||
Phytomedicine Evodiamine inhibits ESCC by inducing M-phase cell-cycle arrest via CUL4A/p53/p21 axis and activating noxa-dependent intrinsic and DR4-dependent extrinsic apoptosis |