TRAPPC9/NIBP Polyclonal antibody

TRAPPC9/NIBP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19549-1-AP

Synonyms

IBP, IKBKBBP, KIAA1882, NIBP, NIK and IKBKB binding protein, T1, TRAPPC9, TRS120, Tularik gene 1 protein

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Validation Data Gallery

• 

  WB analysis of SH-SY5Y using 19549-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19549-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 19549-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 19549-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 19549-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 19549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 19549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, human brain tissue, mouse kidney tissue
• Positive IHC detected in: human heart tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19549-1-AP targets TRAPPC9/NIBP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: trafficking protein particle complex 9
• Calculated Molecular Weight: 139 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: NM_031466
• Gene Symbol: TRAPPC9
• Gene ID (NCBI): 83696
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAPPC9, also named NIK- and IKBKB-binding protein (NIBP), is involved in the NF-kappaB signaling pathway and directly interacts with IKK-beta and MAP3K14. TRAPPC9 functions as an activator of NF-kappa-B via increased phosphorylation of the IKK complex. TARPPC9 may also function in neuronal cells differentiation and defects in TRAPPC9 are the cause of mental retardation autosomal recessive type 13 (MRT13), which is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior. TARPPC9 is a component of the multisubunit TRAPP (transport protein particle) complex and it may play a role in vesicular transport from endoplasmic reticulum to Golgi.