TRPC4AP Polyclonal antibody
TRPC4AP Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC,ELISA
Cat no : 12606-1-AP
Validation Data Gallery
|Positive WB detected in||human heart tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12606-1-AP targets TRPC4AP in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TRPC4AP fusion protein Ag3299|
|Full Name||transient receptor potential cation channel, subfamily C, member 4 associated protein|
|Calculated molecular weight||797 aa, 91 kDa|
|Observed molecular weight||91 kDa|
|GenBank accession number||BC013144|
|Gene ID (NCBI)||26133|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
TRPC4AP, also named as C20orf188 and TRRP4AP, is a substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. TRPC4AP participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. The calculated molecular weight of TRPC4AP is 91 kDa. Western blot analysis detected a protein of about 82 kDa expressed in several mouse cell lines and in a human promonocytic cell line.
|Product Specific Protocols|
|WB protocol for TRPC4AP antibody 12606-1-AP||Download protocol|
|IHC protocol for TRPC4AP antibody 12606-1-AP||Download protocol|
|IP protocol for TRPC4AP antibody 12606-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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