TRPC4AP Polyclonal antibody

TRPC4AP Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 12606-1-AP

Synonyms

C20orf188, Protein TAP1, Protein TRUSS, Trp4 associated protein, Trpc4 associated protein, TRPC4AP, TRRP4AP, TRUSS



Tested Applications

Positive WB detected inhuman heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

12606-1-AP targets TRPC4AP in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPC4AP fusion protein Ag3299
Full Name transient receptor potential cation channel, subfamily C, member 4 associated protein
Calculated molecular weight 797 aa, 91 kDa
Observed molecular weight 91 kDa
GenBank accession numberBC013144
Gene symbol TRPC4AP
Gene ID (NCBI) 26133
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRPC4AP, also named as C20orf188 and TRRP4AP, is a substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. TRPC4AP participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. The calculated molecular weight of TRPC4AP is 91 kDa. Western blot analysis detected a protein of about 82 kDa expressed in several mouse cell lines and in a human promonocytic cell line.

Protocols

Product Specific Protocols
WB protocol for TRPC4AP antibody 12606-1-APDownload protocol
IHC protocol for TRPC4AP antibody 12606-1-APDownload protocol
IP protocol for TRPC4AP antibody 12606-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell Cycle

The G1 phase E3 ubiquitin ligase TRUSS that gets deregulated in human cancers is a novel substrate of the S-phase E3 ubiquitin ligase Skp2.

Authors - Azfar Jamal
humanWB

Oncogene

PP2A-B56α controls oncogene-induced senescence in normal and tumor human melanocytic cells.

Authors - Mannava S S
WB

Genes Dev

Myc protein is stabilized by suppression of a novel E3 ligase complex in cancer cells.

Authors - Seung H Choi