TRPV6 Polyclonal antibody

TRPV6 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 13411-1-AP

Synonyms

CATL, CaT1, CaT like, CaT L, Calcium transport protein 1

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Validation Data Gallery

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  WB analysis of human placenta using 13411-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13411-1-AP (TRPV6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13411-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13411-1-AP (TRPV6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13411-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13411-1-AP (TRPV6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13411-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13411-1-AP (TRPV6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 13411-1-AP

  IP result of anti-TRPV6 (IP:13411-1-AP, 4ug; Detection:13411-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

• 

  IF Staining of HeLa using 13411-1-AP

  Immunofluorescent analysis of Hela cells, using TRPV6 antibody 13411-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse kidney tissue, PC-3 cells
• Positive IP detected in: mouse kidney tissue
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IF: See 5 publications below

Product Information

13411-1-AP targets TRPV6 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRPV6 fusion protein Ag4060
• Full Name: transient receptor potential cation channel, subfamily V, member 6
• Calculated Molecular Weight: 725 aa, 83 kDa
• Observed Molecular Weight: 83 kDa, 70 kDa
• GenBank Accession Number: BC034814
• Gene Symbol: TRPV6
• Gene ID (NCBI): 55503
• RRID: AB_2272390
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPV6, also named as CAT1 or ECaC2, is a member of the transient receptor potential (TRP) family of membrane proteins. Unlike most TRP channels, TRPV6 and its closest relative, TRPV5, are calcium-selective channels. TRPV6 is highly expressed in the proximal intestine, placenta and exocrine tissues (PMID: 12869611). It is probably involved in calcium absorption in various tissues, including calcium reabsorption in kidney. TRPV6 is overexpressed in some cancers and exhibits oncogenic potential.

Publications

Species	Application	Title
human	WB	J Mol Cell Cardiol; TRPV6 protects ER stress-induced apoptosis via ATF6α-TRPV6-JNK pathway in human embryonic stem cell-derived cardiomyocytes.; Authors - Zhichao Li
human	IF	iScience; A microengineered Brain-Chip to model neuroinflammation in humans; Authors - Iosif Pediaditakis
human	WB,IF	Mol Cancer Res; PMID: 19996302; Authors - Bolanz Katrin A KA
mouse	WB	Front Pharmacol; Aqueous Extract of Mori Folium Exerts Bone Protective Effect Through Regulation of Calcium and Redox Homeostasis via PTH/VDR/CaBP and AGEs/RAGE/Nox4/NF-κB Signaling in Diabetic Rats.; Authors - Chenyue Liu
human	WB	J Gastroenterol Hepatol; Vitamin D receptor is overexpressed in the duodenum of patients with irritable bowel syndrome.; Authors - Ko Miura
mouse	WB	Mol Reprod Dev; Proteomic analysis of seminal extracellular vesicle proteins involved in asthenozoospermia by iTRAQ.; Authors - Yu Lin

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