TRPV6 Polyclonal antibody

TRPV6 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 13411-1-AP

Synonyms

ABP/ZF, Calcium transport protein 1, CaT L, CaT like, CAT1, CATL, ECAC2, Epithelial calcium channel 2, HSA277909, LP6728, TRPV6, ZFAB



Tested Applications

Positive WB detected inhuman placenta tissue, mouse kidney tissue, PC-3 cells
Positive IP detected inmouse kidney tissue
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13411-1-AP targets TRPV6 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRPV6 fusion protein Ag4060
Full Name transient receptor potential cation channel, subfamily V, member 6
Calculated molecular weight 725 aa, 83 kDa
Observed molecular weight 83 kDa, 70 kDa
GenBank accession numberBC034814
Gene symbol TRPV6
Gene ID (NCBI) 55503
RRIDAB_2272390
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRPV6, also named as CAT1 or ECaC2, is a member of the transient receptor potential (TRP) family of membrane proteins. Unlike most TRP channels, TRPV6 and its closest relative, TRPV5, are calcium-selective channels. TRPV6 is highly expressed in the proximal intestine, placenta and exocrine tissues (PMID: 12869611). It is probably involved in calcium absorption in various tissues, including calcium reabsorption in kidney. TRPV6 is overexpressed in some cancers and exhibits oncogenic potential.

Protocols

Product Specific Protocols
WB protocol for TRPV6 antibody 13411-1-APDownload protocol
IF protocol for TRPV6 antibody 13411-1-APDownload protocol
IP protocol for TRPV6 antibody 13411-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Mol Cell Cardiol

TRPV6 protects ER stress-induced apoptosis via ATF6α-TRPV6-JNK pathway in human embryonic stem cell-derived cardiomyocytes.

Authors - Zhichao Li
humanIF

iScience

A microengineered Brain-Chip to model neuroinflammation in humans

Authors - Iosif Pediaditakis
humanWB,IF

Mol Cancer Res

PMID: 19996302

Authors - Bolanz Katrin A KA
mouseWB

Front Pharmacol

Aqueous Extract of Mori Folium Exerts Bone Protective Effect Through Regulation of Calcium and Redox Homeostasis via PTH/VDR/CaBP and AGEs/RAGE/Nox4/NF-κB Signaling in Diabetic Rats.

Authors - Chenyue Liu
humanWB

J Gastroenterol Hepatol

Vitamin D receptor is overexpressed in the duodenum of patients with irritable bowel syndrome.

Authors - Ko Miura
mouseWB

Mol Reprod Dev

Proteomic analysis of seminal extracellular vesicle proteins involved in asthenozoospermia by iTRAQ.

Authors - Yu Lin