TSEN15 Polyclonal antibody, PBS Only

• 

  WB analysis using 26054-1-AP (same clone as 26054-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26054-1-AP (TSEN15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26054-1-PBS in a different storage buffer formulation.

TSEN15 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 26054-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

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Product Information

26054-1-PBS targets TSEN15 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22082

  Product name: Recombinant human TSEN15 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-171 aa of BC022030

  Sequence: MEERGDSEPTPGCSGLGPGGVRGFGDGGGAPSWAPEDAWMGTHPKYLEMMELDIGDATQVYVAFLVYLDLMESKSWHEVNCVGLPELQLICLVGTEIEGEGLQTVVPTPITASLSHNRIREILKASRKLQGDPDLPMSFTLAIVESDSTIVYYKLTDGFMLPDPQNISLRR

• Full Name: tRNA splicing endonuclease 15 homolog (S. cerevisiae)
• Calculated Molecular Weight: 171 aa, 19 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC022030
• Gene Symbol: TSEN15
• Gene ID (NCBI): 116461
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WW01
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TSEN15 is the non-catalytic subunit of the tRNA-splicing endonuclease complex, which is a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini (PMID: 15109492, 27392077).