TSPAN31 Polyclonal antibody

TSPAN31 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21987-1-AP

Synonyms

Sarcoma amplified sequence, SAS, tetraspanin 31, Tspan 31, TSPAN31

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Validation Data Gallery

• 

  WB analysis of HeLa using 21987-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21987-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 21987-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 21987-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21987-1-AP (TSPAN31 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 21987-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 21987-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 21987-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 21987-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21987-1-AP (TSPAN31 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, mouse skeletal muscle tissue, PC-3 cells
• Positive IHC detected in: human skeletal muscle tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

The immunogen of 21987-1-AP is TSPAN31 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TSPAN31 fusion protein Ag17138
• Full Name: tetraspanin 31
• Calculated Molecular Weight: 210 aa, 23 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC010377
• Gene Symbol: TSPAN31
• Gene ID (NCBI): 6302
• RRID: AB_2878962
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tetraspanin-31 (TSPAN31) belongs to the tetraspanin (TM4SF) family, also known as the transmembrane 4 (TM4) superfamily, which are a family of transmembrane proteins with 33 mammalian members. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. Tetraspanin-31 is thought to be involved in growth-related cellular processes. The experimentally determined molecular mass of 30 kDa is larger than the calculated molecular mass of 23 kDa, which could be a result of post-translational modifications.

Publications

Species	Application	Title
human	IHC	Cancer Sci; Overexpression of Tetraspanin31 contributes to malignant potential and poor outcomes in gastric cancer; Authors - Yusuke Takashima