Validation Data Gallery
|Positive WB detected in||fetal human brain tissue, SH-SY5Y cells|
|Positive IHC detected in||human cerebellum tissue, mouse brain tissue, mouse cerebellum tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue, iPS cells, rat brain tissue|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66375-1-Ig targets TUBB3-specific in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Full Name||tubulin, beta 3|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||NM_001197181|
|Gene ID (NCBI)||10381|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms.
Biochem Biophys Res Commun
Functional analysis of juxta- and intra-membrane domains of murine APP by genome editing in Neuro2a cells.
Metab Brain Dis
Taurine improves the differentiation of neural stem cells in fetal rats with intrauterine growth restriction via activation of the PKA-CREB-BDNF signaling pathway.
Stem Cells Dev
Zinc Promotes Patient-Derived Induced Pluripotent Stem Cell Neural Differentiation via ERK-STAT Signaling.
CNS Neurosci Ther
A single factor induces neuronal differentiation to suppress glioma cell growth.
Front Cell Neurosci
The Neural Stem Cell Properties of PKD2L1+ Cerebrospinal Fluid-Contacting Neurons in vitro.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Josh (Verified Customer) (12-18-2018)
BIII tubulin antibody signal nicely fills neuronal cell bodies and processes with good signal to noise at a reasonable dilution (1:500 in 1% NDS, 0.3% Triton-X, PBS with donkey anti-mouse 647 secondary, 1:500). However, BIII tubulin antibody signal also colocalized with S100 immunoreactive astrocytic processes, though with a lesser intensity. Overall, the antibody is a useful marker of neuronal differentiation when used in combination with other cell-selective antibodies and morphology. Image caption: Differentiated adult hippocampal neural stem cell culture. BIII tubulin 1:500 (purple), S100 1:500 (red), Hoechst (blue). Scale bar = 20 um.