TUBB3-specific Monoclonal antibody
TUBB3-specific Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1F8G10
Cat no : 66375-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | fetal human brain tissue, SH-SY5Y cells |
| Positive IHC detected in | human cerebellum tissue, mouse brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse brain tissue, iPS cells, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 3 publications below |
| IF | See 5 publications below |
| FC | See 1 publications below |
Product Information
66375-1-Ig targets TUBB3-specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | tubulin, beta 3 |
| Calculated molecular weight | 55 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | NM_001197181 |
| Gene symbol | TUBB3 |
| Gene ID (NCBI) | 10381 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TUBB3-specific antibody 66375-1-Ig | Download protocol |
| IHC protocol for TUBB3-specific antibody 66375-1-Ig | Download protocol |
| IF protocol for TUBB3-specific antibody 66375-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun Functional analysis of juxta- and intra-membrane domains of murine APP by genome editing in Neuro2a cells. | ||
Stem Cells Dev Zinc Promotes Patient-Derived Induced Pluripotent Stem Cell Neural Differentiation via ERK-STAT Signaling. | ||
CNS Neurosci Ther A single factor induces neuronal differentiation to suppress glioma cell growth. | ||
Cell Physiol Biochem A Human Long Non-Coding RNA ALT1 Controls the Cell Cycle of Vascular Endothelial Cells Via ACE2 and Cyclin D1 Pathway. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Josh (Verified Customer) (12-18-2018) | BIII tubulin antibody signal nicely fills neuronal cell bodies and processes with good signal to noise at a reasonable dilution (1:500 in 1% NDS, 0.3% Triton-X, PBS with donkey anti-mouse 647 secondary, 1:500). However, BIII tubulin antibody signal also colocalized with S100 immunoreactive astrocytic processes, though with a lesser intensity. Overall, the antibody is a useful marker of neuronal differentiation when used in combination with other cell-selective antibodies and morphology. Image caption: Differentiated adult hippocampal neural stem cell culture. BIII tubulin 1:500 (purple), S100 1:500 (red), Hoechst (blue). Scale bar = 20 um.
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