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TUBB3-specific Antibody 4 Publications

Mouse Monoclonal| Catalog number: 66375-1-Ig |CloneNo.: 1F8G10

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
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Images See all

TUBB3-specific Antibody IF iPS cells 66375-1-Ig

Immunofluorescent staining of TUBB3 (66375-1-lg, 1:250) with 4% PFA fixed control hiPSC derived neuronal cultures (35 days old). (Green: TUBB3; Blue: DAPI). Provided by BioTalentum Ltd., Hungary.

TUBB3-specific Antibody IHC mouse brain tissue 66375-1-Ig

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

TUBB3-specific Antibody IF mouse brain tissue 66375-1-Ig

Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

TUBB3-specific Antibody WB fetal human brain tissue 66375-1-Ig

Western blot analysis of TUBB3 in various tissues and cell lines with 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:40,000 incubated at room temperature for 1.5 hours

TUBB3-specific Antibody IHC human cerebellum tissue 66375-1-Ig

Immunohistochemistry of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:400 (under 40x lens).

TUBB3-specific Antibody IHC human cerebellum tissue 66375-1-Ig

Immunohistochemistry of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:400 (under 10x lens).

TUBB3-specific Antibody WB SH-SY5Y cells 66375-1-Ig

Western blot analysis of TUBB3 in various cell lines with 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:40,000 incubated at room temperature for 1.5 hours

Species specificity:
human, mouse, rat
Positive WB detected in:
fetal human brain tissue, SH-SY5Y cells
Positive IHC detected in:
human cerebellum tissue, mouse brain tissue, mouse cerebellum tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse brain tissue, iPS cells
Recommended dilution:
WB : 1:5000-1:50000
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
tubulin, beta 3
Calculated molecular weight:
55
Observed molecular weight:
50-55 kDa
GenBank accession number:
NM_001197181
Gene ID (NCBI):
10381
Gene symbol
TUBB3
Synonyms
beta 4, beta tubulin, MC1R, TUBB, TUBB3, TUBB3-specific, Tubulin beta 3 chain, Tubulin beta III, tubulin, beta 3, Tuj1

Background

TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

TUBB3-specific Mouse Monoclonal antibody

Josh (Verified Customer), 12-18-2018
Applications
Immunofluorescence,Cell culture,
Primary Antibody Dilution
1:500
TUBB3-specific Antibody Immunofluorescence,Cell culture, validation (1:500 dilution) in Adult hippocampal neural stem cells, differentiated for 7 days (Cat no:66375-1-Ig)

TUBB3-specific Antibody Immunofluorescence,Cell culture, validation (1:500 dilution) in Adult hippocampal neural stem cells, differentiated for 7 days (Cat no:66375-1-Ig)

Cell/Tissue Type
Adult hippocampal neural stem cells, differentiated for 7 days
Lot Number
BIII tubulin antibody signal nicely fills neuronal cell bodies and processes with good signal to noise at a reasonable dilution (1:500 in 1% NDS, 0.3% Triton-X, PBS with donkey anti-mouse 647 secondary, 1:500). However, BIII tubulin antibody signal also colocalized with S100 immunoreactive astrocytic processes, though with a lesser intensity. Overall, the antibody is a useful marker of neuronal differentiation when used in combination with other cell-selective antibodies and morphology. Image caption: Differentiated adult hippocampal neural stem cell culture. BIII tubulin 1:500 (purple), S100 1:500 (red), Hoechst (blue). Scale bar = 20 um.

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