TUBB3-specific/TUJ1 Monoclonal antibody

TUBB3-specific/TUJ1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat, rabbit, chicken, pig







Cat no : 66375-1-Ig


beta 4, beta tubulin, MC1R, TUBB, TUBB3, TUBB3-specific, Tubulin beta 3 chain, Tubulin beta III, tubulin, beta 3, Tuj1, tuj1 antibody

Tested Applications

Positive WB detected inSH-SY5Y cells, PC-12 cells, Neuro-2a cells, Pig brain, Rabbit brain, Rat brain Mouse brain, Chicken brain, human brain tissue
Positive IHC detected inhuman cerebellum tissue, mouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue, Retinal organoids, iPS cells, mouse brain tissue, human neuron

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:20000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66375-1-Ig targets TUBB3-specific/TUJ1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, rabbit, chicken, pig samples.

Tested Reactivity human, mouse, rat, rabbit, chicken, pig
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name tubulin, beta 3
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberNM_001197181
Gene Symbol TUBB3
Gene ID (NCBI) 10381
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms.


Product Specific Protocols
WB protocol for TUBB3-specific/TUJ1 antibody 66375-1-IgDownload protocol
IHC protocol for TUBB3-specific/TUJ1 antibody 66375-1-IgDownload protocol
IF protocol for TUBB3-specific/TUJ1 antibody 66375-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Gabriele (Verified Customer) (01-12-2023)

The antibody works very well with our IF protocol. Maybe the dilution could be pushed to 1:500-1:600. TUBB3 1:400 (green), Hoechst (Blue)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: hiPSC-derived Neurons
TUBB3-specific/TUJ1 Antibody Immunofluorescence validation (1:400 dilution) in hiPSC-derived Neurons (Cat no:66375-1-Ig)

Alessandro (Verified Customer) (07-27-2022)

No aspecific staining, great outcome

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: ROs

Josh (Verified Customer) (12-18-2018)

BIII tubulin antibody signal nicely fills neuronal cell bodies and processes with good signal to noise at a reasonable dilution (1:500 in 1% NDS, 0.3% Triton-X, PBS with donkey anti-mouse 647 secondary, 1:500). However, BIII tubulin antibody signal also colocalized with S100 immunoreactive astrocytic processes, though with a lesser intensity. Overall, the antibody is a useful marker of neuronal differentiation when used in combination with other cell-selective antibodies and morphology. Image caption: Differentiated adult hippocampal neural stem cell culture. BIII tubulin 1:500 (purple), S100 1:500 (red), Hoechst (blue). Scale bar = 20 um.

  • Applications: Immunofluorescence, Cell culture,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Adult hippocampal neural stem cells, differentiated for 7 days
TUBB3-specific/TUJ1 Antibody Immunofluorescence,Cell culture, validation (1:500 dilution) in Adult hippocampal neural stem cells, differentiated for 7 days (Cat no:66375-1-Ig)