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TUBB3-specific Antibody 2 Publications

Mouse Monoclonal| Catalog number: 66375-1-Ig |CloneNo.: 1F8G10

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

TUBB3-specific Antibody IF iPS cells 66375-1-Ig

Immunofluorescent staining of TUBB3 (66375-1-lg, 1:250) with 4% PFA fixed control hiPSC derived neuronal cultures (35 days old). (Green: TUBB3; Blue: DAPI). Provided by BioTalentum Ltd., Hungary.

TUBB3-specific Antibody WB fetal human brain tissue 66375-1-Ig

Western blot analysis of TUBB3 in various tissues and cell lines with 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:40,000 incubated at room temperature for 1.5 hours

TUBB3-specific Antibody IHC human cerebellum tissue 66375-1-Ig

Immunohistochemistry of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:400 (under 40x lens).

TUBB3-specific Antibody IHC human cerebellum tissue 66375-1-Ig

Immunohistochemistry of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:400 (under 10x lens).

TUBB3-specific Antibody WB SH-SY5Y cells 66375-1-Ig

Western blot analysis of TUBB3 in various cell lines with 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:40,000 incubated at room temperature for 1.5 hours

TUBB3-specific Antibody IF mouse brain tissue 66375-1-Ig

Immunofluorescent analysis of ( 4% PFA ) fixed mouse brain tissue using 66375-1-Ig(TUBB3-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
fetal human brain tissue, SH-SY5Y cells
Positive IHC detected in:
human cerebellum tissue, mouse cerebellum tissue
Positive IF detected in:
mouse brain tissue, iPS cells
Recommended dilution:
WB : 1:5000-1:50000
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
tubulin, beta 3
Calculated molecular weight:
55
Observed molecular weight:
50-55 kDa
GenBank accession number:
NM_001197181
Gene ID (NCBI):
10381
Gene symbol
TUBB3
Synonyms
beta 4, beta tubulin, MC1R, TUBB, TUBB3, TUBB3-specific, Tubj1, Tubulin beta 3 chain, Tubulin beta III, tubulin, beta 3

Background

TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms.


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