TUBB3-specific Monoclonal antibody
TUBB3-specific Monoclonal Antibody for FC, IHC, WB, ELISA
Cat no : 66375-1-Ig
Validation Data Gallery
|Positive WB detected in||SH-SY5Y cells, PC-12 cells, Neuro-2a cells, Pig brain, Rabbit brain, Rat brain Mouse brain, Chicken brain, human brain tissue|
|Positive IHC detected in||human cerebellum tissue, mouse brain tissue, mouse cerebellum tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:400-1:20000|
|Sample-dependent, check data in validation data gallery|
66375-1-Ig targets TUBB3-specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, rabbit, chicken, pig samples.
|Tested Reactivity||human, mouse, rat, rabbit, chicken, pig|
|Cited Reactivity||human, mouse, rat, chicken|
|Host / Isotype||Mouse / IgG1|
|Full Name||tubulin, beta 3|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||NM_001197181|
|Gene ID (NCBI)||10381|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms.
Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.
Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation.
Silk fibroin nanofibrous scaffolds incorporated with microRNA-222 loaded chitosan nanoparticles for enhanced neuronal differentiation of neural stem cells.
Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons.
Epigenetic suppression of liver X receptor β in anterior cingulate cortex by HDAC5 drives CFA-induced chronic inflammatory pain.
Front Cell Dev Biol
ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Gabriele (Verified Customer) (01-12-2023)
The antibody works very well with our IF protocol. Maybe the dilution could be pushed to 1:500-1:600. TUBB3 1:400 (green), Hoechst (Blue)
Alessandro (Verified Customer) (07-27-2022)
No aspecific staining, great outcome
Josh (Verified Customer) (12-18-2018)
BIII tubulin antibody signal nicely fills neuronal cell bodies and processes with good signal to noise at a reasonable dilution (1:500 in 1% NDS, 0.3% Triton-X, PBS with donkey anti-mouse 647 secondary, 1:500). However, BIII tubulin antibody signal also colocalized with S100 immunoreactive astrocytic processes, though with a lesser intensity. Overall, the antibody is a useful marker of neuronal differentiation when used in combination with other cell-selective antibodies and morphology. Image caption: Differentiated adult hippocampal neural stem cell culture. BIII tubulin 1:500 (purple), S100 1:500 (red), Hoechst (blue). Scale bar = 20 um.