TUBB3-specific Monoclonal antibody, PBS Only

TUBB3-specific Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit, chicken, pig

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F8G10

Cat no : 66375-1-PBS

Synonyms

beta 4, beta tubulin, MC1R, TUBB, TUBB3, TUBB3-specific, Tubulin beta 3 chain, Tubulin beta III, tubulin, beta 3, Tuj1, tuj1 antibody

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Validation Data Gallery

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  WB analysis using 66375-1-Ig (same clone as 66375-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:49000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66375-1-Ig (same clone as 66375-1-PBS)

  Western blot analysis of TUBB3 in various tissues and cell lines with 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:40,000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66375-1-Ig (same clone as 66375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 66375-1-Ig (same clone as 66375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 66375-1-Ig (same clone as 66375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 66375-1-Ig (same clone as 66375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

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  IHC staining of human cerebellum using 66375-1-Ig (same clone as 66375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 66375-1-Ig (same clone as 66375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 66375-1-Ig (same clone as 66375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66375-1-Ig (same clone as 66375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66375-1-Ig (same clone as 66375-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

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  IF Staining of Retinal organoids using 66375-1-Ig (same clone as 66375-1-PBS)

  Retinal organoids (day 60) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and Cytokeratin 19 using 10712-1-AP at 1:200 (red). Nuclear stain DAPI (blue). Scale bar = 100 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria. This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

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  IF Staining of iPS cells using 66375-1-Ig (same clone as 66375-1-PBS)

  Immunofluorescent staining of TUBB3 (66375-1-lg, 1:250) with 4% PFA fixed control hiPSC derived neuronal cultures (35 days old). (Green: TUBB3; Blue: DAPI). Provided by BioTalentum Ltd., Hungary. This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

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  IF Staining of Retinal organoids using 66375-1-Ig (same clone as 66375-1-PBS)

  Retinal organoids (day 250) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and CRALBP using 15356-1-AP at 1:400 (red). Nuclear stain DAPI (blue). Scale bar = 50 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria. This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

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  IF Staining of human neuron using 66375-1-Ig (same clone as 66375-1-PBS)

  Immunofluorescence analysis of human pluripotent stem cell-derived astrocytes with S100β (15146-1-AP) at 1/200 (Magenta) and neurons with TUJ1 (66375-1-Ig) at 1:500 (Green). The sample was fixed with 4% Paraformaldehyde and permeabilized with 0.3% Triton X-100. Alexa Fluor 488-conjugated goat anti-mouse IgG (1/500) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (1/500) were used as the secondary antibodies. Nuclei were counterstained with DAPI (blue). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

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  IF Staining of Retinal organoids using 66375-1-Ig (same clone as 66375-1-PBS)

  Retinal organoids (day 30) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and PAX6 (12323-1-AP) at 1:500. Nuclear stain DAPI (blue). Scale bar = 50 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria. This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 66375-1-Ig (same clone as 66375-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 66375-1-Ig (TUBB3-specific antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The section was co-stained with 26975-1-AP (NeuN antibody, red). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66375-1-Ig (same clone as 66375-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 66375-1-Ig (same clone as 66375-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66375-1-Ig (TUBB3-specific antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 66375-1-Ig (same clone as 66375-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human TUBB3-specific (66375-1-Ig, Clone:1F8G10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66375-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66375-1-PBS targets TUBB3-specific in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat, rabbit, chicken, pig samples.

• Tested Reactivity: human, mouse, rat, rabbit, chicken, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tubulin, beta 3
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: NM_001197181
• Gene Symbol: TUBB3
• Gene ID (NCBI): 10381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms.