CoraLite® Plus 488-conjugated TUBB3-specific/TUJ1 Monoclonal antibody

TUBB3-specific/TUJ1 Monoclonal Antibody for IF, WB

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F8G10

Cat no : CL488-66375

Synonyms

beta 4, beta tubulin, MC1R, TUBB, TUBB3, TUBB3-specific, TUBB4, Tubulin beta 3 chain, Tubulin beta 4 chain, Tubulin beta III, tubulin, beta 3, tuj1, tuj1 antibody



Tested Applications

Positive WB detected inNeuro-2a cells
Positive IF detected inmouse brain tissue, mouse spine, mouse cerebellum tissue, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66375 targets TUBB3-specific/TUJ1 in WB, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name tubulin, beta 3
Calculated Molecular Weight 55 kDa
GenBank Accession NumberNM_001197181
Gene Symbol TUBB3
Gene ID (NCBI) 10381
RRIDAB_2883321
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms. And the antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 TUBB3-specific/TUJ1 antibody CL488-66375Download protocol
IF protocol for CL Plus 488 TUBB3-specific/TUJ1 antibody CL488-66375Download protocol
Standard Protocols
Click here to view our Standard Protocols