CoraLite® Plus 488-conjugated TUBB3-specific/TUJ1 Monoclonal antibody

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  WB analysis using CL488-66375

  Neuro-2a cell lysates were subjected to SDS PAGE followed by western blot with CL488-66375 (TUBB3-specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using CL488-66375

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66375 (TUBB3-specific antibody) at dilution of 1:50.

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  IF Staining of mouse brain using CL488-66375

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66375 (TUBB3-specific antibody) at dilution of 1:50.

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  IF Staining of mouse spine using CL488-66375

  E14.5 FFPE mouse spine stained for beta-III tubulin (red, Cat. No CL488-66375) and GFAP (cyan, Cat. No 60190-1-Ig). Beta-III tubulin stains neurons and was conjugated to CoraLite-488 fluorescent dye and pseudocolored to red. GFAP stains astrocytes along the spinal column. In this image, astrocyte projections can be seen among spinal neurons in the spinal cord. Image credit: @Immunofluorescence on Instagram.

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  IF Staining of mouse cerebellum using CL488-66375

  Neonatal mouse cerebellum stained for beta-III tubulin (magenta, Cat. No CL488-66375) and GFAP (green, Cat. No 60190-1-Ig). Beta-III tubulin stains neurons and was conjugated to CoraLite-488 fluorescent dye and pseudocolored to magenta. GFAP stains astrocytes. In this image, astrocyte projections can be seen intermingling with neurons. Image credit: @Immunofluorescence on Instagram.

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  IF Staining of mouse testis using CL488-66375

  Adult mouse testes were co-stained with TUBB3 (CL488-66375, in green), DDX4/VASA (51042-1-AP, in magenta), and PCNA (60097-1-Ig, in blue). TUBB3/Beta III tubulin marks the Sertoli cells (structural support cells), VASA marks the germ cells (developing sperm), and PCNA marks proliferating cells (these cells are at the base of the tubule, where the sperm stem cells are located). The image was created in paid partnership with @Immunofluorescence on Instagram.

TUBB3-specific/TUJ1 Monoclonal Antibody for WB, IF-P

Cat No. CL488-66375

Clone No.1F8G10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF-P

Synonyms

TUBB3, TUBB3-specific, TUJ1, beta tubulin, Tubulin beta 3 chain

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Tested Applications

• Positive WB detected in: Neuro-2a cells
• Positive IF-P detected in: mouse brain tissue, mouse spine, mouse cerebellum tissue, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66375 targets TUBB3-specific/TUJ1 in WB, IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tubulin, beta 3
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: NM_001197181
• Gene Symbol: TUBB3
• Gene ID (NCBI): 10381
• RRID: AB_2883321
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q13509
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms. And the antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.