Tested Applications
| Positive WB detected in | SH-SY5Y cells, PC-12 cells, Neuro-2a cells |
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-66375 targets TUBB3-specific/TUJ1 in WB, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | tubulin, beta 3 |
| Calculated Molecular Weight | 55 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | NM_001197181 |
| Gene Symbol | TUBB3 |
| Gene ID (NCBI) | 10381 |
| RRID | AB_2883571 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Excitation Laser | Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13509 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms. And the antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 TUBB3-specific/TUJ1 antibody CL594-66375 | Download protocol |
| IF protocol for CL594 TUBB3-specific/TUJ1 antibody CL594-66375 | Download protocol |
| WB protocol for CL594 TUBB3-specific/TUJ1 antibody CL594-66375 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TUBB3-specific/TUJ1 Monoclonal antibody (1F8G10), catalog number CL594-66375, has 4 citations. These appear in Cell Proliferation, Scientific Reports, Experimental Cell Research, and Hearing Research. The associated research fields span osteogenesis and dental stem cell biology, urinary tract neurotoxicity, salivary gland development, and auditory neuroscience (spiral ganglion neuron autophagy).
| Species | Application | Title |
|---|---|---|
Cell Prolif Collagen Type VI Alpha 1 as a Regulator of Redox Homeostasis in Antioxidant-Enhanced Osteogenesis of Dental Stem Cells. | ||
Sci Rep Sexual dimorphic impacts of systemic vincristine on lower urinary tract function. | ||
Exp Cell Res A parasympathetic neurotransmitter induces myoepithelial cell differentiation during salivary gland development. | ||
Hear Res Noise exposure induces autophagy-modulated nuclear-to-cytoplasmic translocation of TDP-43 in spiral ganglion neurons. |
Reviews
What customers say
The single available review rates the product 4 out of 5 stars. The reviewer used it for immunofluorescence on FA-fixed E3 chick neural tube tissue at a 1:100 dilution and reported good detection of differentiating neurons. Overall, the feedback is positive, highlighting reliable performance in tissue-based IF applications at a standard dilution.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Victoria (Verified Customer) (10-15-2021) | Good IF detection of differentiating neurons in tissue at a concentration of 1:100.
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