TUSC2 Polyclonal antibody

TUSC2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11538-1-AP

Synonyms

C3orf11, Fus 1 protein, FUS1, Fusion 1 protein, LGCC, PAP, PDAP2, PDGFA associated protein 2, tumor suppressor candidate 2, TUSC2

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Validation Data Gallery

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  WB analysis of human heart using 11538-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11538-1-AP (TUSC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 11538-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11538-1-AP (TUSC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11538-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11538-1-AP (TUSC2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 11538-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11538-1-AP (TUSC2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 11538-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11538-1-AP (TUSC2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 11538-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11538-1-AP (TUSC2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 11538-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11538-1-AP (TUSC2 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A549 using 11538-1-AP

  Immunofluorescent analysis of A549 cells using 11538-1-AP (TUSC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human heart tissue, mouse pancreas tissue
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

11538-1-AP targets TUSC2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TUSC2 fusion protein Ag2118
• Full Name: tumor suppressor candidate 2
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 10-12 kDa
• GenBank Accession Number: BC023976
• Gene Symbol: TUSC2
• Gene ID (NCBI): 11334
• RRID: AB_10699869
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FUS1 (or TUSC2) gene is a highly conserved lung cancer candidate gene, which was identified in the 120 kb 3p21.3 critical region contained in nested lung and breast cancer homozygous deletions. Overexpression of FUS1 gene leads to G1 arrest and growth inhibition of lung cancer cells (PMID: 11593436). The encoded Fusion-1 protein was down-regulated, mutated or lost in the majority of inflammatory thoracic malignancies. It has been evidenced that Fusion-1 establishes its immune- and tumour-suppressive activities via regulation of mitochondrial homeostasis (PMID: 22513871). In addition, myristoylation is found to be required for Fusion-1-mediated tumor-suppressing activity and suggest a novel mechanism for the inactivation of tumor suppressors in lung cancer and a role for deficient posttranslational modification in tumor suppressor-gene-mediated carcinogenesis (PMID: 15126327).

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Cancer Lett; NEDD4 degrades TUSC2 to promote glioblastoma progression.; Authors - Tadas K Rimkus
human	WB,IHC	Oncotarget; TWIST1/miR-584/TUSC2 pathway induces resistance to apoptosis in thyroid cancer cells.; Authors - Francesca Maria Orlandella