TRX2 Polyclonal antibody

• Featured Product
• KD/KO Validated

TRX2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

30

Cat no : 13089-1-AP

Synonyms

Thioredoxin-2, Thioredoxin, mitochondrial, Thioredoxin 2, Mt-Trx, MTRX

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Validation Data Gallery

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  WB analysis of Raji using 13089-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13089-1-AP (TRX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13089-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13089-1-AP (TRX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 13089-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13089-1-AP (TRX2 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: Raji cells, human liver tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 23 publications below
• IHC: See 3 publications below
• IF: See 5 publications below

Product Information

13089-1-AP targets TRX2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TRX2 fusion protein Ag3640
• Full Name: thioredoxin 2
• Calculated Molecular Weight: 166 aa, 18 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC013726
• Gene Symbol: Thioredoxin 2
• Gene ID (NCBI): 25828
• RRID: AB_2304119
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TXN2 (Thioredoxin-2) is also named as TRX2, MTRX, Mt-TRX and regulates the mitochondrial redox state and plays an important role in cell proliferation. It has been shown that cells conditionally deficient in Trx2 undergo apoptosis in the absence of exogenous stress, accompanied by an accumulation of intracellular ROS, the activation of caspase 9 and caspase 3, and the release of cytochrome c into the cytosol. In the retina, the expression profile and the role of Trx2 have not yet been defined (PMID: 18441302).

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer; Authors - Rong Wang
mouse	WB	Nat Commun; G6PD protects from oxidative damage and improves healthspan in mice.; Authors - Sandrina Nóbrega-Pereira
mouse	WB	Cell Rep; The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.; Authors - Wu Hongtan H
mouse	WB	Mol Ther Nucleic Acids; Diverging targets mediate the pathological roleof miR-199a-5p and miR-199a-3p by promoting cardiac hypertrophy and fibrosis; Authors - Ni Zeng
human	WB	Int J Mol Sci; TRX2/Rab35 Interaction Impairs Exosome Secretion by Inducing Rab35 Degradation.; Authors - Tao Zhang ; KD Validated
human	WB	Free Radic Biol Med; Oxidative stress as a candidate mechanism for accelerated neuroectodermal differentiation due to trisomy 21.; Authors - Kendra M Prutton