Tie-2/CD202b Recombinant antibody, PBS Only

• 

  WB analysis of human placenta using 86010-2-RR (same clone as 86010-2-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 86010-2-RR (TEK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86010-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HUVEC using 86010-2-RR (same clone as 86010-2-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 86010-2-RR (TEK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86010-2-PBS in a different storage buffer formulation.

Tie-2/CD202b Recombinant Antibody for WB, Indirect ELISA

Cat No. 86010-2-PBS

Clone No.250234F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

TEK, Angiopoietin 1 receptor, Angiopoietin-1 receptor, CD202B, EC:2.7.10.1

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Product Information

86010-2-PBS targets Tie-2/CD202b in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Tie-2/CD202b fusion protein Eg3367
• Full Name: TEK tyrosine kinase, endothelial
• Calculated Molecular Weight: 1124 aa, 126 kDa
• Observed Molecular Weight: 120 kDa, 160 kDa
• GenBank Accession Number: BC035514
• Gene Symbol: Tie2
• Gene ID (NCBI): 7010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q02763
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Tie2 (also known as TEK) is a tyrosine-protein kinase expressed almost exclusively on endothelial cells. It contains two immunoglobulin-like domains, three epidermal growth factor (EGF)--like domains and three fibronectin type III repeats. Tie2 acts as a cell-surface receptor for ANGPT1, ANGPT2, and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Mutations in the gene Tie2 are associated with inherited venous malformations of the skin and mucous membranes. Human Tie2 has a calculated molecular weight of 126 kDa. As a result of glycosylation, the apparent molecular mass of Tie2 is approximately 140-160 kDa.