Tie-2/CD202b Recombinant antibody

• 

  WB analysis of human placenta using 86010-2-RR

  human placenta tissue were subjected to SDS PAGE followed by western blot with 86010-2-RR (TEK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 86010-2-RR

  HUVEC cells were subjected to SDS PAGE followed by western blot with 86010-2-RR (TEK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tie-2/CD202b Recombinant Antibody for WB, ELISA

Cat No. 86010-2-RR

Clone No.250234F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

TEK, Angiopoietin 1 receptor, Angiopoietin-1 receptor, CD202B, EC:2.7.10.1

---

Tested Applications

• Positive WB detected in: human placenta tissue, HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86010-2-RR targets Tie-2/CD202b in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Tie-2/CD202b fusion protein Eg3367
• Full Name: TEK tyrosine kinase, endothelial
• Calculated Molecular Weight: 1124 aa, 126 kDa
• Observed Molecular Weight: 120 kDa, 160 kDa
• GenBank Accession Number: BC035514
• Gene Symbol: Tie2
• Gene ID (NCBI): 7010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q02763
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tie2 (also known as TEK) is a tyrosine-protein kinase expressed almost exclusively on endothelial cells. It contains two immunoglobulin-like domains, three epidermal growth factor (EGF)--like domains and three fibronectin type III repeats. Tie2 acts as a cell-surface receptor for ANGPT1, ANGPT2, and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Mutations in the gene Tie2 are associated with inherited venous malformations of the skin and mucous membranes. Human Tie2 has a calculated molecular weight of 126 kDa. As a result of glycosylation, the apparent molecular mass of Tie2 is approximately 140-160 kDa.