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  • Western Blot (WB) analysis of human placenta tissue using Tie-2/CD202b Recombinant antibody (86010-2-RR)

    WB analysis of human placenta using 86010-2-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 86010-2-RR (TEK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HUVEC cells using Tie-2/CD202b Recombinant antibody (86010-2-RR)

    WB analysis of HUVEC using 86010-2-RR

    HUVEC cells were subjected to SDS PAGE followed by western blot with 86010-2-RR (TEK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tie-2/CD202b Recombinant antibody

Tie-2/CD202b Recombinant Antibody for WB, ELISA

Cat No. 86010-2-RR
Clone No.250234F8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

TEK, Angiopoietin 1 receptor, Angiopoietin-1 receptor, CD202B, EC:2.7.10.1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inhuman placenta tissue, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86010-2-RR targets Tie-2/CD202b in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Tie-2/CD202b fusion protein Eg3367 Predict reactive species
Full Name TEK tyrosine kinase, endothelial
Calculated Molecular Weight 1124 aa, 126 kDa
Observed Molecular Weight120 kDa, 160 kDa
GenBank Accession NumberBC035514
Gene Symbol Tie2
Gene ID (NCBI) 7010
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ02763
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tie2 (also known as TEK) is a tyrosine-protein kinase expressed almost exclusively on endothelial cells. It contains two immunoglobulin-like domains, three epidermal growth factor (EGF)--like domains and three fibronectin type III repeats. Tie2 acts as a cell-surface receptor for ANGPT1, ANGPT2, and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Mutations in the gene Tie2 are associated with inherited venous malformations of the skin and mucous membranes. Human Tie2 has a calculated molecular weight of 126 kDa. As a result of glycosylation, the apparent molecular mass of Tie2 is approximately 140-160 kDa.

Protocols

Product Specific Protocols
WB protocol for Tie-2/CD202b antibody 86010-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols