UAP1L1 Polyclonal antibody

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  WB analysis of MDA-MB-453s using 25262-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 25262-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25262-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25262-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 25262-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25262-1-AP (UAP1L1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 25262-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25262-1-AP (UAP1L1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 25262-1-AP

  Immunofluorescent analysis of HepG2 cells using 25262-1-AP (UAP1L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

UAP1L1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25262-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:2.7.7.-, UDP-N-acetylhexosamine pyrophosphorylase-like protein 1

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Tested Applications

• Positive WB detected in: MDA-MB-453s cells, A375 cells, HepG2 cells, K-562 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25262-1-AP targets UAP1L1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: UDP-N-acteylglucosamine pyrophosphorylase 1-like 1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57-59 kDa
• GenBank Accession Number: NM_207309
• Gene Symbol: UAP1L1
• Gene ID (NCBI): 91373
• RRID: AB_2879993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q3KQV9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UDP-N-acetylglucosamine pyrophosphorylase-1-like-1 (UAP1L1) is a protein that shares ~59% sequence identity with UDP-N-acetylglucosamine pryophosphorylase-1 (UAP1), which is directly involved in the synthesis of the sugar donor (UDP-GlcNac) for N-acetylglucosamine modification (O-GlcNAclation) of proteins in the cytoplasm, nucleus, and mitochondria, as well as for O- and N-linked protein glycosylation in the endoplasmic reticulum and the Golgi apparatus (PMID: 30097606, 35168617). Human UAP1L1 is also significantly upregulated in a distinct subset of HCC tissues and patients with upregulated expression of UAP1L1 appeared to have poor prognosis (PMID: 33987240).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Heliyon; Changes in transcriptomic landscape with macronutrients intake switch are independent from O-GlcNAcylation levels in heart throughout postnatal development in rats; Authors - Antoine Persello