UBL3 Polyclonal antibody

UBL3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14100-1-AP

Synonyms

HCG 1, HsMUB, MUB, PNSC1, Protein HCG 1, ubiquitin like 3, Ubiquitin like protein 3, UBL3

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Validation Data Gallery

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  WB analysis using 14100-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14100-1-AP (UBL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14100-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14100-1-AP (UBL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Caco-2 cells, human brain tissue, U-87 MG cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14100-1-AP targets UBL3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBL3 fusion protein Ag5233
• Full Name: ubiquitin-like 3
• Calculated Molecular Weight: 117 aa, 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC059385
• Gene Symbol: UBL3
• Gene ID (NCBI): 5412
• RRID: AB_2212060
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin-like 3 (UBL3)/membrane-anchored Ub-fold protein (MUB) acts as a post-translational modification (PTM) factor to regulate efficient protein sorting to sEVs (small extracellular vesicles). (PMID: 31363817, PMID: 30258067).