UBQLN2 Polyclonal antibody

UBQLN2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 23449-1-AP

Synonyms

CHAP1, CHAP1/DSK2, Dsk2, DSK2 homolog, hPLIC 2, HRIHFB2157, LIC 2, N4BP4, PLIC 2, PLIC2, RIHFB2157, ubiquilin 2, Ubiquilin-2, UBQLN2



Tested Applications

Positive WB detected inrat brain tissue, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23449-1-AP is UBQLN2 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBQLN2 fusion protein Ag20149
Full Name ubiquilin 2
Calculated molecular weight 624 aa, 66 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC069237
Gene symbol UBQLN2
Gene ID (NCBI) 29978
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UBQLN2 antibody 23449-1-APDownload protocol
IHC protocol for UBQLN2 antibody 23449-1-APDownload protocol
IF protocol for UBQLN2 antibody 23449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

J Neurochem

Pathogenic mutation of UBQLN2 impairs its interaction with UBXD8 and disrupts endoplasmic reticulum-associated protein degradation.

Authors - Yuxing Xia
mouseIF

Brain Pathol

Serpin neuropathology in the P497S UBQLN2 mouse model of ALS/FTD.

Authors - Nicole R Higgins

Hum Mol Genet

Conditional depletion of intellectual disability and Parkinsonism candidate gene ATP6AP2 in fly and mouse induces cognitive impairment and neurodegeneration.

Authors - Aline Dubos