Ubiquilin 2 Polyclonal antibody

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• Ubiquilin 2 Polyclonal antibody (23449-1-AP)

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• KD/KO Validated

Ubiquilin 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 23449-1-AP

Publications(13)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

UBQLN2, ALS15, Chap1, Dsk2, hPLIC 2

• 

  WB analysis of rat brain using 23449-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23449-1-AP (UBQLN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 23449-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23449-1-AP (Ubiquilin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 23449-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23449-1-AP (UBQLN2 antibody at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 23449-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23449-1-AP (UBQLN2 antibody at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 23449-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23449-1-AP (UBQLN2 Antibody) at dilution of 1:50.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 23449-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23449-1-AP (UBQLN2 Antibody) at dilution of 1:50.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 23449-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UBQLN2 antibody (23449-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, rat brain tissue, HeLa cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23449-1-AP targets Ubiquilin 2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20149

  Product name: Recombinant human Ubiquilin 2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-624 aa of BC069237

  Sequence: MAENGESSGPPRPSRGPAAAQGSAAAPAEPKIIKVTVKTPKEKEEFAVPENSSVQQFKEAISKRFKSQTDQLVLIFAGKILKDQDTLIQHGIHDGLTVHLVIKSQNRPQGQSTQPSNAAGTNTTSASTPRSNSTPISTNSNPFGLGSLGGLAGLSSLGLSSTNFSELQSQMQQQLMASPEMMIQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHLLNNPDIMRQTLEIARNPAMMQEMMRNQDLALSNLESIPGGYNALRRMYTDIQEPMLNAAQEQFGGNPFASVGSSSSSGEGTQPSRTENRDPLPNPWAPPPATQSSATTSTTTSTGSGSGNSSSNATGNTVAAANYVASIFSTPGMQSLLQQITENPQLIQNMLSAPYMRSMMQSLSQNPDLAAQMMLNSPLFTANPQLQEQMRPQLPAFLQQMQNPDTLSAMSNPRAMQALMQIQQGLQTLATEAPGLIPSFTPGVGVGVLGTAIGPVGPVTPIGPIGPIVPFTPIGPIGPIGPTGPAAPPGSTGSGGPTGPTVSSAAPSETTSPTSESGPNQQFIQQMVQALAGANAPQLPNPEVRFQQQLEQLNAMGFLNREANLQALIATGGDINAAIERLLGSQPS

• Full Name: ubiquilin 2
• Calculated Molecular Weight: 624 aa, 66 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC069237
• Gene Symbol: UBQLN2
• Gene ID (NCBI): 29978
• RRID: AB_2879282
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UHD9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquilin-2 (UBQLN2) is a multifunctional, ubiquitin-binding shuttle protein that plays a central role in maintaining cellular protein homeostasis. It is a member of the ubiquilin family of proteins found in all eukaryotes, which function to regulate the degradation of ubiquitinated proteins. Of the five different UBQLN genes in humans, UBQLN2 is the most highly expressed in the nervous system and muscle tissue and has been linked to multiple neurodegenerative diseases. (PMID: 40663766, PMID: 40663766)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Brain; Tamoxifen improves muscle structure and function of Bin1- and Dnm2-related centronuclear myopathies; Authors - Charlotte Gineste
		Hum Mol Genet; Conditional depletion of intellectual disability and Parkinsonism candidate gene ATP6AP2 in fly and mouse induces cognitive impairment and neurodegeneration.; Authors - Aline Dubos
mouse	IF	Brain Pathol; Serpin neuropathology in the P497S UBQLN2 mouse model of ALS/FTD.; Authors - Nicole R Higgins
	WB	J Neurochem; Pathogenic mutation of UBQLN2 impairs its interaction with UBXD8 and disrupts endoplasmic reticulum-associated protein degradation.; Authors - Yuxing Xia
	WB	bioRxiv; Short N-terminal disordered regions and the proline-rich domain are major regulators of phase transitions for full-length UBQLN1, UBQLN2 and UBQLN4; Authors - Thuy P Dao
human	WB	Biophys J; Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation; Authors - Thuy P Dao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Xingyuan (Verified Customer) (06-22-2023)

Antibody quality was good.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

Ubiquilin 2 Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:23449-1-AP)