UBQLN2 Polyclonal antibody

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  WB analysis of rat brain using 23449-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23449-1-AP (UBQLN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23449-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23449-1-AP (UBQLN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 23449-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23449-1-AP (UBQLN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 23449-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23449-1-AP (UBQLN2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 23449-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23449-1-AP (UBQLN2 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 23449-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23449-1-AP (UBQLN2 Antibody) at dilution of 1:50.

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  IHC staining of human testis using 23449-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23449-1-AP (UBQLN2 Antibody) at dilution of 1:50.

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  IF Staining of HepG2 using 23449-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UBQLN2 antibody (23449-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

UBQLN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 23449-1-AP

Publications(9)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CHAP1, CHAP1/DSK2, Dsk2, DSK2 homolog, hPLIC 2, HRIHFB2157, LIC 2, N4BP4, PLIC 2, PLIC2, RIHFB2157, ubiquilin 2, Ubiquilin-2, UBQLN2

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Tested Applications

• Positive WB detected in: rat brain tissue, HEK-293 cells, HepG2 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Product Information

23449-1-AP targets UBQLN2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBQLN2 fusion protein Ag20149
• Full Name: ubiquilin 2
• Calculated Molecular Weight: 624 aa, 66 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC069237
• Gene Symbol: UBQLN2
• Gene ID (NCBI): 29978
• RRID: AB_2879282
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UHD9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Brain; Tamoxifen improves muscle structure and function of Bin1- and Dnm2-related centronuclear myopathies; Authors - Charlotte Gineste
		Hum Mol Genet; Conditional depletion of intellectual disability and Parkinsonism candidate gene ATP6AP2 in fly and mouse induces cognitive impairment and neurodegeneration.; Authors - Aline Dubos
mouse	IF	Brain Pathol; Serpin neuropathology in the P497S UBQLN2 mouse model of ALS/FTD.; Authors - Nicole R Higgins
	WB	J Neurochem; Pathogenic mutation of UBQLN2 impairs its interaction with UBXD8 and disrupts endoplasmic reticulum-associated protein degradation.; Authors - Yuxing Xia
human	WB,IF,IP	F1000Res; The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence; Authors - Ian McDowell ; KO Validated
	WB	bioRxiv; Short N-terminal disordered regions and the proline-rich domain are major regulators of phase transitions for full-length UBQLN1, UBQLN2 and UBQLN4; Authors - Thuy P Dao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xingyuan (Verified Customer) (06-22-2023)

Antibody quality was good.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

UBQLN2 Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:23449-1-AP)