UBQLN4 Polyclonal antibody

UBQLN4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29413-1-AP

Synonyms

A1U, C1orf6, CIP75, UBIN, ubiquilin 4, UBQLN4

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Validation Data Gallery

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  WB analysis using 29413-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29413-1-AP (UBQLN4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 29413-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29413-1-AP (UBQLN4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 29413-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29413-1-AP (UBQLN4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 29413-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 29413-1-AP (UBQLN4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: BGC-823 cells, BxPC-3 cells, HeLa cells, MKN-45 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29413-1-AP targets UBQLN4 in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBQLN4 fusion protein Ag29658
• Full Name: ubiquilin 4
• Calculated Molecular Weight: 601 aa, 64 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC063841
• Gene Symbol: UBQLN4
• Gene ID (NCBI): 56893
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquilins (UBQLNs) are important factors for cell proteostasis maintenance. UBQLNs are involved in the modulation of the cell cycle, as well as in apoptosis, membrane receptors regulation, DNA repair, epithelial-mesenchymal transition, and miRNA activities. Ubiquilin 4 (UBQLN4) is an important member of the ubiquitin-like protein family. UBQLN4 is overexpressed in aggressive tumors and inhibits homologous recombination, redirecting doublestrand break repair to nonhomologous end joining, resulting in increased genome instability and inducing carcinogenesis.