UIMC1 Polyclonal antibody, PBS Only

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  WB analysis of mouse colon using 13642-1-AP (same clone as 13642-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13642-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13642-1-AP (same clone as 13642-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13642-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 13642-1-AP (same clone as 13642-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13642-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 13642-1-AP (same clone as 13642-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13642-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 13642-1-AP (same clone as 13642-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13642-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 13642-1-AP (same clone as 13642-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 13642-1-AP (UIMC1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13642-1-PBS in a different storage buffer formulation.

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  IP experiment of COLO 320 using 13642-1-AP (same clone as 13642-1-PBS)

  IP result of anti-UIMC1 (IP:13642-1-AP, 4ug; Detection:13642-1-AP 1:500) with COLO 320 cells lysate 2400ug. This data was developed using the same antibody clone with 13642-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 13642-1-AP (same clone as 13642-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13642-1-AP (UIMC1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13642-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 13642-1-AP (same clone as 13642-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13642-1-AP (UIMC1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13642-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13642-1-AP (same clone as 13642-1-PBS)

  Immunofluorescent analysis of Hela cells, using UIMC1 antibody 13642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 13642-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13642-1-AP (same clone as 13642-1-PBS)

  Immunofluorescent analysis of Hela cells, using UIMC1 antibody 13642-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 13642-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 13642-1-AP (same clone as 13642-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using UIMC1 antibody 13642-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 13642-1-PBS in a different storage buffer formulation.

UIMC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 13642-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

BRCA1-A complex subunit RAP80, RAP80, Receptor-associated protein 80, Retinoid X receptor-interacting protein 110, RXRIP110

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Product Information

13642-1-PBS targets UIMC1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4582

  Product name: Recombinant human UIMC1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 202-553 aa of BC032561

  Sequence: DQSSQPVFENVNVKSFDRCTGHSAEHTQCGKPQSSQGIVEETSEEGNSVPASQSVAALTSKRSLVLMPESSAEEITVCPETQLSSSETFDLEREVSPGSRDILDGVRIIMADKEVGNKEDAEKEVAISTFSSSNQVSCPLCDQCFPPTKIERHAMYCNGLMEEDTVLTRRQKEAKTKSDSGTAAQTSLDIDKNEKCYLCKSLVPFREYQCHVDSCLQLAKADQGDGPEGSGRACSTVEGKWQQRLKNPKEKGHSEGRLLSFLEQSEHKTSDADIKSSETGAFRVPSPGMEEAGCSREMQSSFTRRDLNESPVKSFVSISEATDCLVDFKKQVTVQPGSRTRTKAGRGRRRKF

• Full Name: ubiquitin interaction motif containing 1
• Calculated Molecular Weight: 719 aa, 80 kDa
• Observed Molecular Weight: 97 kDa, 80 kDa, 71-75 kDa, 61 kDa
• GenBank Accession Number: BC032561
• Gene Symbol: UIMC1
• Gene ID (NCBI): 51720
• RRID: AB_10641052
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96RL1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

UIMC1, also known as RAP80 (Receptor-Associated Protein 80), is a nuclear protein that plays a critical role in the DNA damage response pathway. UIMC1 serves as a key scaffold protein that recruits the BRCA1-A complex (including BRCA1, BRCC36, and Abraxas) to damaged chromatin, thereby facilitating homologous recombination-mediated repair and maintaining genomic stability.