UPK1A Polyclonal antibody

UPK1A Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25275-1-AP

Synonyms

Tetraspanin 21, Tspan 21, TSPAN21, UP1A, UPIA, UPK1A, UPKA, uroplakin 1A, Uroplakin Ia

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Validation Data Gallery

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  IP experiment of mouse bladder using 25275-1-AP

  IP result of anti-UPK1A (IP:25275-1-AP, 4ug; Detection:25275-1-AP 1:300) with mouse bladder tissue lysate 3200ug.

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  IHC staining of mouse bladder using 25275-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 25275-1-AP (UPK1A antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse bladder using 25275-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 25275-1-AP (UPK1A antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat bladder using 25275-1-AP

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 25275-1-AP (UPK1A antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat bladder using 25275-1-AP

  Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 25275-1-AP (UPK1A antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bladder using 25275-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 25275-1-AP (UPK1A antibody) at dilution of 1:5000 (under 10x lens)..

• 

  IHC staining of human bladder using 25275-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 25275-1-AP (UPK1A antibody) at dilution of 1:5000 (under 40x lens)..

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Tested Applications

• Positive IP detected in: mouse bladder tissue
• Positive IHC detected in: human bladder tissue, rat bladder tissue, mouse bladder tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 25275-1-AP is UPK1A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UPK1A fusion protein Ag17356
• Full Name: uroplakin 1A
• Calculated Molecular Weight: 258 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC107098
• Gene Symbol: UPK1A
• Gene ID (NCBI): 11045
• RRID: AB_2880000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin-1a (UPK1A) belongs to the tetraspanin (TM4SF) family. UPK1A plays an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane.

Publications

Species	Application	Title
human	IF	Stem Cells Int; Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.; Authors - Cong Li
human	IHC	Int J Urol; Differential effects of adipose tissue stromal cells on the apoptosis, growth and invasion of bladder urothelial carcinoma between the superficial and invasive types.; Authors - Maki Kawasaki-Nanri