URB1-Specific Polyclonal antibody

URB1-Specific Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20023-1-AP

Synonyms

C21orf108, KIAA0539, URB1

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Validation Data Gallery

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  WB analysis of K-562 using 20023-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20023-1-AP (URB1-Specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 20023-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20023-1-AP (URB1-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

20023-1-AP targets URB1-Specific in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: URB1 ribosome biogenesis 1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 254 kDa
• Observed Molecular Weight: 240-260 kDa
• GenBank Accession Number: NM_014825
• Gene Symbol: URB1
• Gene ID (NCBI): 9875
• RRID: AB_10667000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

URB1, also named as C21orf108, KIAA0539 and NPA1, is a 2,271 amino acid protein that is homologous to the S. cerevisiae protein URB1 which is a nucleolar protein involved in the normal accumulation of 25S and 5.8S rRNAs and may also play a role in the biosynthesis of the 60S ribosomal unit. Also localized to the nucleolus, human URB1 is thought to have the same functions as S. cerevisiae URB1. The antibody is specific to URB1.

Publications

Species	Application	Title
human	WB	Nature; Nucleolar URB1 ensures 3' ETS rRNA removal to prevent exosome surveillance; Authors - Lin Shan ; KD Validated