Filter: AllWBIPIHCIF/ICC

WB analysis using 22340-1-AP (same clone as 22340-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 22340-1-AP (same clone as 22340-1-PBS)
WB result of USP11 antibody (22340-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP11 transfected HEK-293 cells. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 22340-1-AP (same clone as 22340-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 22340-1-AP (same clone as 22340-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 22340-1-AP (same clone as 22340-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.
WB analysis of human testis using 22340-1-AP (same clone as 22340-1-PBS)
human testis tissue were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.
WB analysis of human testis using 22340-1-AP (same clone as 22340-1-PBS)
human testis tissue were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 22340-1-AP (same clone as 22340-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 22340-1-AP (same clone as 22340-1-PBS)
IP result of anti-USP11 (IP:22340-1-AP, 4ug; Detection:22340-1-AP 1:2000) with HEK-293 cells lysate 2400ug. This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 22340-1-AP (same clone as 22340-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 22340-1-AP (same clone as 22340-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 22340-1-AP (same clone as 22340-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 22340-1-AP (same clone as 22340-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 22340-1-AP (same clone as 22340-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 22340-1-AP (same clone as 22340-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 22340-1-AP (same clone as 22340-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 22340-1-AP (same clone as 22340-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.
IF Staining of HEK-293 using 22340-1-AP (same clone as 22340-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using USP11 antibody (22340-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22340-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

USP11 Polyclonal antibody, PBS Only

USP11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 22340-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Deubiquitinating enzyme 11, EC:3.4.19.12, Ubiquitin carboxyl-terminal hydrolase 11, Ubiquitin thioesterase 11, Ubiquitin thiolesterase 11

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

22340-1-PBS targets USP11 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17829 Product name: Recombinant human USP11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 441-743 aa of BC000350 Sequence: YLSVPLLISHKRVLEVFFIPMDPRRKPEQHRLVVPKKGKISDLCVALSKHTGISPERMMVADVFSHRFYKLYQLEEPLSSILDRDDIFVYEVSGRIEAIEGSREDIVVPVYLRERTPARDYNNSYYGLMLFGHPLLVSVPRDRFTWEGLYNVLMYRLSRYVTKPNSDDEDDGDEKEDDEEDKDDVPGPSTGGSLRDPEPEQAGPSSGVTNRCPFLLDNCLGTSQWPPRRRRKQLFTLQTVNSNGTSDRTTSPEEVHAQPYIAIDWEPEMKKRYYDEVEAEGYVKHDCVGYVMKKAPVRLQECI Predict reactive species
Full Name	ubiquitin specific peptidase 11
Calculated Molecular Weight	963 aa, 110 kDa
Observed Molecular Weight	110 kDa
GenBank Accession Number	BC000350
Gene Symbol	USP11
Gene ID (NCBI)	8237
RRID	AB_2879079
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P51784
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

USP11(Ubiquitin carboxyl-terminal hydrolase 11) is also named as UHX1 and belongs to the peptidase C19 family. It is a deubiquitinating enzyme that controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction.