USP11 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP11 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 22340-1-AP

Synonyms

Deubiquitinating enzyme 11, Ubiquitin thiolesterase 11, UHX1, USP11

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Validation Data Gallery

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  WB analysis using 22340-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22340-1-AP

  WB result of USP11 antibody (22340-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP11 transfected HEK-293 cells.

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  WB analysis of A549 using 22340-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 22340-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 22340-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22340-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22340-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 22340-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 22340-1-AP (USP11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 22340-1-AP

  IP result of anti-USP11 (IP:22340-1-AP, 4ug; Detection:22340-1-AP 1:2000) with HEK-293 cells lysate 2400ug.

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  IHC staining of human breast cancer using 22340-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22340-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 22340-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 22340-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22340-1-AP (USP11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 22340-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 22340-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 22340-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 22340-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22340-1-AP (USP11 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 22340-1-AP

  Immunofluorescent analysis of HEK-293 cells using 22340-1-AP (USP11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, A549 cells, HEK-293 cells, human testis tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human brain tissue, human liver cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

22340-1-AP targets USP11 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP11 fusion protein Ag17829
• Full Name: ubiquitin specific peptidase 11
• Calculated Molecular Weight: 963 aa, 110 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC000350
• Gene Symbol: USP11
• Gene ID (NCBI): 8237
• RRID: AB_2879079
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP11(Ubiquitin carboxyl-terminal hydrolase 11) is also named as UHX1 and belongs to the peptidase C19 family. It is a deubiquitinating enzyme that controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction.

Publications

Species	Application	Title
human	WB,IHC	Lab Invest; Ubiquitin-specific protease 11 serves as a marker of poor prognosis and promotes metastasis in hepatocellular carcinoma.; Authors - Sheng Zhang ; KD Validated
	WB	Cell Rep; TRIM32/USP11 Balances ARID1A Stability and the Oncogenic/Tumor-Suppressive Status of Squamous Cell Carcinoma.; Authors - Qingyu Luo
human	WB,IF	Cell Death Discov; Knockdown of RRM1 in tumor cells promotes radio-/chemotherapy induced ferroptosis by regulating p53 ubiquitination and p21-GPX4 signaling axis.; Authors - Yang Gao
human	WB,IF	Cell; X-linked ubiquitin-specific peptidase 11 increases tauopathy vulnerability in women; Authors - Yan Yan ; KD Validated
human	WB	Sci Adv; Oncogenic deubiquitination controls tyrosine kinase signaling and therapy response in acute lymphoblastic leukemia; Authors - Qi Jin ; KD Validated
human	IHC,IF	Cell Death Dis; USP11-mediated LSH deubiquitination inhibits ferroptosis in colorectal cancer through epigenetic activation of CYP24A1; Authors - Junyi Duan