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  • KD/KO Validated

USP11 Polyclonal antibody

USP11 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22340-1-AP

Synonyms

Deubiquitinating enzyme 11, Ubiquitin thiolesterase 11, UHX1, USP11



Tested Applications

Positive WB detected inJurkat cells, A549 cells, HEK-293 cells, human testis tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human brain tissue, human liver cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

22340-1-AP targets USP11 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP11 fusion protein Ag17829
Full Name ubiquitin specific peptidase 11
Calculated molecular weight 963 aa, 110 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC000350
Gene symbol USP11
Gene ID (NCBI) 8237
RRIDAB_2879079
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP11(Ubiquitin carboxyl-terminal hydrolase 11) is also named as UHX1 and belongs to the peptidase C19 family. It is a deubiquitinating enzyme that controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction.

Protocols

Product Specific Protocols
WB protocol for USP11 antibody 22340-1-APDownload protocol
IHC protocol for USP11 antibody 22340-1-APDownload protocol
IF protocol for USP11 antibody 22340-1-APDownload protocol
IP protocol for USP11 antibody 22340-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Lab Invest

Ubiquitin-specific protease 11 serves as a marker of poor prognosis and promotes metastasis in hepatocellular carcinoma.

Authors - Sheng Zhang
  • KD Validated
WB

Cell Rep

TRIM32/USP11 Balances ARID1A Stability and the Oncogenic/Tumor-Suppressive Status of Squamous Cell Carcinoma.

Authors - Qingyu Luo
humanWB,IF

Cell Death Discov

Knockdown of RRM1 in tumor cells promotes radio-/chemotherapy induced ferroptosis by regulating p53 ubiquitination and p21-GPX4 signaling axis.

Authors - Yang Gao
humanWB,IF

Cell

X-linked ubiquitin-specific peptidase 11 increases tauopathy vulnerability in women

Authors - Yan Yan
  • KD Validated
humanWB

Sci Adv

Oncogenic deubiquitination controls tyrosine kinase signaling and therapy response in acute lymphoblastic leukemia

Authors - Qi Jin
  • KD Validated
humanIHC,IF

Cell Death Dis

USP11-mediated LSH deubiquitination inhibits ferroptosis in colorectal cancer through epigenetic activation of CYP24A1

Authors - Junyi Duan