CoraLite®594-conjugated USP4 Monoclonal antibody

USP4 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G11G8

Cat no : CL594-66822

Synonyms

Deubiquitinating enzyme 4, Ubiquitin thiolesterase 4, UNP, Unph, USP4

---

Validation Data Gallery

• 

  FC experiment of THP-1 using CL594-66822

  1X10^6 THP-1 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human USP4 (CL594-66822, Clone:1G11G8) (red), or 0.8 ug CoraLite®594-conjugated Mouse IgG2b isotype control Mouse McAb (CL594-66360-3, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive FC detected in: THP-1 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66822 targets USP4 in FC (Intra) applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: USP4 fusion protein Ag28297
• Full Name: ubiquitin specific peptidase 4 (proto-oncogene)
• Calculated Molecular Weight: 963 aa, 109 kDa
• Observed Molecular Weight: 109 kDa
• GenBank Accession Number: BC125131
• Gene Symbol: USP4
• Gene ID (NCBI): 7375
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.