Validation Data Gallery
|Positive WB detected in||Jurkat cells, K-562 cells, HL-60 cells|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human breast cancer tissue, human brain tissue, human kidney tissue, human liver tissue, human heart tissue, human spleen tissue, human lung tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16364-1-AP targets VAV1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, rat, gecko|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VAV1 fusion protein Ag9049|
|Full Name||vav 1 guanine nucleotide exchange factor|
|Calculated molecular weight||845 aa, 98 kDa|
|Observed molecular weight||98 kDa|
|GenBank accession number||BC013361|
|Gene ID (NCBI)||7409|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
J Biol Chem
Adult astrocytes from reptiles are resistant to proinflammatory activation via sustaining Vav1 expression.
Biochem Biophys Res Commun
Vav1 expression is increased in esophageal squamous cell carcinoma and indicates poor prognosis.
Multiple regulatory aspects of histone methyltransferase EZH2 in Pb-induced neurotoxicity.
Clin Sci (Lond)
Novel role of Vav1-Rac1 pathway in actin cytoskeleton regulation in Interleukin-13-induced minimal change-like nephropathy.
Cancer Cell Int
STING pathway contributes to the prognosis of hepatocellular carcinoma and identification of prognostic gene signatures correlated to tumor microenvironment
Neural Regen Res
Vav1 promotes inflammation and neuronal apoptosis in cerebral ischemia/reperfusion injury by upregulating microglial and NLRP3 inflammasome activation