VAV1 Polyclonal antibody

• Featured Product
• KD/KO Validated

VAV1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 16364-1-AP

Synonyms

VAV, Proto-oncogene vav, Proto oncogene vav

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Validation Data Gallery

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  WB analysis of Jurkat using 16364-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16364-1-AP (VAV1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 16364-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16364-1-AP (VAV1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16364-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16364-1-AP (VAV1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16364-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16364-1-AP (VAV1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 16364-1-AP

  IP result of anti-VAV1 (IP:16364-1-AP, 4ug; Detection:16364-1-AP 1:500) with K-562 cells lysate 1200ug.

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  IHC staining of human breast cancer using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16364-1-AP (VAV1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lymphoma using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 16364-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 16364-1-AP (VAV1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, HL-60 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human breast cancer tissue, human brain tissue, human kidney tissue, human liver tissue, human heart tissue, human spleen tissue, human lung tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

16364-1-AP targets VAV1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat, gecko
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VAV1 fusion protein Ag9049
• Full Name: vav 1 guanine nucleotide exchange factor
• Calculated Molecular Weight: 845 aa, 98 kDa
• Observed Molecular Weight: 98 kDa
• GenBank Accession Number: BC013361
• Gene Symbol: VAV1
• Gene ID (NCBI): 7409
• RRID: AB_2213571
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vav proteins mainly act as enzymes that catalyze the activation step of Rho subfamily GTPases during cell signaling. There are three family members:VAV1, VAV2 and VAV3. Vav1 is specifically expressed in the hematopoietic system, whereas Vav2 and Vav3 are more ubiquitously expressed (PMID:14607270). Vav1 is physiologically active as a GDP/GTP nucleotide exchange factor (GEF) in the hematopoietic system (PMID:31654719).

Publications

Species	Application	Title
gecko	WB,IF	J Biol Chem; Adult astrocytes from reptiles are resistant to proinflammatory activation via sustaining Vav1 expression.; Authors - Nan Du ; KD Validated
human	WB,IHC	Biochem Biophys Res Commun; Vav1 expression is increased in esophageal squamous cell carcinoma and indicates poor prognosis.; Authors - Xiaolei Zhu
human	WB	Oncotarget; Multiple regulatory aspects of histone methyltransferase EZH2 in Pb-induced neurotoxicity.; Authors - Wei-Zhen Xue
rat	WB,IF	Clin Sci (Lond); Novel role of Vav1-Rac1 pathway in actin cytoskeleton regulation in Interleukin-13-induced minimal change-like nephropathy.; Authors - Chang-Yien Chan ; KD Validated
human	WB	Cancer Cell Int; STING pathway contributes to the prognosis of hepatocellular carcinoma and identification of prognostic gene signatures correlated to tumor microenvironment; Authors - Zhangya Pu
rat	IF	Neural Regen Res; Vav1 promotes inflammation and neuronal apoptosis in cerebral ischemia/reperfusion injury by upregulating microglial and NLRP3 inflammasome activation; Authors - Jing Qiu