Validation Data Gallery
|Positive WB detected in||Jurkat cells, K-562 cells, HL-60 cells|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human breast cancer tissue, human brain tissue, human kidney tissue, human liver tissue, human heart tissue, human spleen tissue, human lung tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16364-1-AP targets VAV1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, rat, gecko|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VAV1 fusion protein Ag9049|
|Full Name||vav 1 guanine nucleotide exchange factor|
|Calculated molecular weight||845 aa, 98 kDa|
|Observed molecular weight||98 kDa|
|GenBank accession number||BC013361|
|Gene ID (NCBI)||7409|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Clin Sci (Lond)
Novel role of Vav1-Rac1 pathway in actin cytoskeleton regulation in Interleukin-13-induced minimal change-like nephropathy.
Multiple regulatory aspects of histone methyltransferase EZH2 in Pb-induced neurotoxicity.
Biochem Biophys Res Commun
Vav1 expression is increased in esophageal squamous cell carcinoma and indicates poor prognosis.
J Biol Chem
Adult astrocytes from reptiles are resistant to proinflammatory activation via sustaining Vav1 expression.