Anti-Mouse VE-cadherin/CD144 Rabbit Recombinant Antibody

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  FC experiment of bEnd.3 using 98251-1-RR

  1x10^6 bEnd.3 cells (left) or C2C12 (right) were surface stained with 0.25 ug Anti-Mouse VE-cadherin/CD144 Rabbit Recombinant Antibody (98251-1-RR, Clone: 241953D4) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98251-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98251-1-RR against Mouse VE-cadherin/CD144 were performed. The affinity constant is 1.65 nM.

VE-cadherin/CD144 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98251-1-RR

Clone No.241953D4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

VE-cadherin, VE Cad, Cdh5, Cd144, cadherin 5

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Tested Applications

• Positive FC detected in: bEnd.3 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98251-1-RR targets VE-cadherin/CD144 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: cadherin 5
• Calculated Molecular Weight: 88kDa
• GenBank Accession Number: NM_009868.4
• Gene Symbol: Cdh5
• Gene ID (NCBI): 12562
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P55284
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609).