VEGFC Polyclonal antibody

VEGFC Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22601-1-AP

Synonyms

Flt4 L, Flt4 ligand, VEGF C, VEGFC, VRP



Tested Applications

Positive WB detected inMCF-7 cells, RAW 264.7 cells
Positive IHC detected inhuman prostate cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22601-1-AP is VEGFC Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VEGFC fusion protein Ag18182
Full Name vascular endothelial growth factor C
Calculated molecular weight 419 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC035212
Gene symbol VEGFC
Gene ID (NCBI) 7424
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for VEGFC antibody 22601-1-APDownload protocol
IHC protocol for VEGFC antibody 22601-1-APDownload protocol
IF protocol for VEGFC antibody 22601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

J Extracell Vesicles

DUSP2 regulates extracellular vesicle-VEGF-C secretion and pancreatic cancer early dissemination.

Authors - Chu-An Wang
mouseWB

Clin Transl Med

VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis.

Authors - Qiu-Yue Lin
humanWB, IHC, IF

Cancer Lett

The Hippo-TAZ axis mediates vascular endothelial growth factor C in glioblastoma-derived exosomes to promote angiogenesis.

Authors - Zhengmin Wang
humanWB

Aging (Albany NY)

MicroRNA-330-3p promotes brain metastasis and epithelial-mesenchymal transition via GRIA3 in non-small cell lung cancer.

Authors - Chunhua Wei
humanWB,IHC

Front Oncol

The Specifically Androgen-Regulated Gene (SARG) Promotes Papillary Thyroid Carcinoma (PTC) Lymphatic Metastasis Through Vascular Endothelial Growth Factor C (VEGF-C) and VEGF Receptor 3 (VEGFR-3) Axis.

Authors - Shuai-Jun Xu
mouse,humanWB

Fluids Barriers CNS

ROS attenuates TET2-dependent ZO-1 epigenetic expression in cerebral vascular endothelial cells

Authors - Lan Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

hongxuan (Verified Customer) (11-12-2018)

we can detect the obvious signals in the embryo sections by using this primary antibody at a dilution of 1:50. Moreover, the background is very clear. Please forgive me that I cannot upload the files due to it's unpublished data.Thank you very much for you product.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: mouse embryo