VEGFC Polyclonal antibody

VEGFC Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22601-1-AP

Synonyms

Flt4 L, Flt4 ligand, VEGF C, VEGFC, VRP



Tested Applications

Positive WB detected inMCF-7 cells, RAW 264.7 cells
Positive IHC detected inhuman prostate cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22601-1-AP is VEGFC Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VEGFC fusion protein Ag18182
Full Name vascular endothelial growth factor C
Calculated molecular weight 419 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC035212
Gene symbol VEGFC
Gene ID (NCBI) 7424
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for VEGFC antibody 22601-1-APDownload protocol
IHC protocol for VEGFC antibody 22601-1-APDownload protocol
IF protocol for VEGFC antibody 22601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cancer

Epigenetically upregulated oncoprotein PLCE1 drives esophageal carcinoma angiogenesis and proliferation via activating the PI-PLCε-NF-κB signaling pathway and VEGF-C/ Bcl-2 expression.

Authors - Yunzhao Chen
humanWB

Theranostics

Transcriptional Downregulation of miR-4306 serves as a New Therapeutic Target for Triple Negative Breast Cancer.

Authors - Zitong Zhao
mouseWB

Oxid Med Cell Longev

Angiotensin II Induces Cardiac Edema and Hypertrophic Remodeling through Lymphatic-Dependent Mechanisms.

Authors - Jie Bai
mouseWB

Cell Reprogram

Wnt Signaling Regulates the Lymphatic Endothelial Transdifferentiation of Adipose-Derived Stromal Cells In Vitro.

Authors - Nian Zhang
humanIHC

Eur J Histochem

Immunoreactivity and a new staining method of monocarboxylate transporter 1 located in endothelial cells of cerebral vessels of human brain in distinguishing cerebral venules from arterioles.

Authors - Yuan Cao
humanWB

PLoS One

ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer.

Authors - Lingjia Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

hongxuan (Verified Customer) (11-12-2018)

we can detect the obvious signals in the embryo sections by using this primary antibody at a dilution of 1:50. Moreover, the background is very clear. Please forgive me that I cannot upload the files due to it's unpublished data.Thank you very much for you product.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: mouse embryo