VEGFC Polyclonal antibody

VEGFC Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 22601-1-AP

Synonyms

Flt4 L, Flt4 ligand, VEGF C, VEGFC, VRP



Tested Applications

Positive WB detected inMCF-7 cells, RAW 264.7 cells
Positive IHC detected inhuman prostate cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22601-1-AP is VEGFC Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VEGFC fusion protein Ag18182
Full Name vascular endothelial growth factor C
Calculated molecular weight 419 aa, 47 kDa
Observed molecular weight 47-50 kDa
GenBank accession numberBC035212
Gene symbol VEGFC
Gene ID (NCBI) 7424
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for VEGFC antibody 22601-1-APDownload protocol
IHC protocol for VEGFC antibody 22601-1-APDownload protocol
IF protocol for VEGFC antibody 22601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cancer

Epigenetically upregulated oncoprotein PLCE1 drives esophageal carcinoma angiogenesis and proliferation via activating the PI-PLCε-NF-κB signaling pathway and VEGF-C/ Bcl-2 expression.

Authors - Yunzhao Chen
humanWB

Theranostics

Transcriptional Downregulation of miR-4306 serves as a New Therapeutic Target for Triple Negative Breast Cancer.

Authors - Zitong Zhao
mouseWB

Cell Reprogram

Wnt Signaling Regulates the Lymphatic Endothelial Transdifferentiation of Adipose-Derived Stromal Cells In Vitro.

Authors - Nian Zhang
humanWB

PLoS One

ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer.

Authors - Lingjia Liu
mouseIHC,IF

Am J Pathol

Macrophages Regulate Unilateral Ureteral Obstruction-Induced Renal Lymphangiogenesis through C-C Motif Chemokine Receptor 2-Dependent Phosphatidylinositol 3-Kinase-AKT-Mechanistic Target of Rapamycin Signaling and Hypoxia-Inducible Factor-1α/Vascular Endothelial Growth Factor-C Expression.

Authors - Yan-Chao Guo
humanIHC

J Cancer

Development and Validation of a Nomogram based on cell growth-related Biomarkers for Oral Squamous Cell Carcinoma.

Authors - Yanjie Shuai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

hongxuan (Verified Customer) (11-12-2018)

we can detect the obvious signals in the embryo sections by using this primary antibody at a dilution of 1:50. Moreover, the background is very clear. Please forgive me that I cannot upload the files due to it's unpublished data.Thank you very much for you product.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: mouse embryo